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CoraLite® Plus 647-conjugated CITED1 Polyclonal antibody

CITED1 Polyclonal Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, rat, mouse

Applications

FC (Intra)

Conjugate

CoraLite® Plus 647 Fluorescent Dye

Cat no : CL647-26999

Print datasheet

Synonyms

CITED1, Melanocyte specific protein 1, MSG1

验证数据展示

  • FC experiment of HEK-293 using CL647-26999

    FC experiment of HEK-293 using CL647-26999

    1X10^6 HEK-293 cells were intracellularly stained with 0.2 ug CoraLite® Plus 647 Anti-Human CITED1 (CL647-26999) (red), or 0.2 ug isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive FC (Intra) detected inHEK-293 cells
Positive FC detected inHEK-293 cells
Planning an IHC experiment? We recommend our IHCeasy CITED1 Ready-To-Use IHC Kit. CITED1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 26999-1-AP

推荐稀释比

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-26999 targets CITED1 in FC (Intra) applications and shows reactivity with human, rat, mouse samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity human, rat, mouse
Immunogen CITED1 fusion protein Ag24643 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name Cbp/p300-interacting transactivator, with Glu/Asp-rich carboxy-terminal domain, 1
Synonyms CITED1, Melanocyte specific protein 1, MSG1
Calculated Molecular Weight 27 kDa
Observed Molecular Weight 30 kDa
GenBank Accession NumberBC004240
Gene Symbol CITED1
Gene ID (NCBI) 4435
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission Maxima Wavelengths654 nm / 674 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ99966
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CITED1, also named as MSG1, belongs to the CITED family. CITED1 is a bifunctional transcriptional cofactor which is able to activate or repress transcription in association with other transcription factors. CITED1 has 2 isoforms with the molecular mass of 20 and 23 kDa. The elevation of CITED1 may be directly associated with the preneoplastic phenotype (PMID: 23935526).

实验方案

Product Specific Protocols
FC protocol for CL Plus 647 CITED1 antibody CL647-26999Download protocol
Standard Protocols
Click here to view our Standard Protocols