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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 84485-4-RR (same clone as 84485-4-PBS)

    WB analysis using 84485-4-RR (same clone as 84485-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84485-4-RR (CKAP4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84485-4-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 84485-4-RR (same clone as 84485-4-PBS)

    IF Staining of HepG2 using 84485-4-RR (same clone as 84485-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CKAP4 antibody (84485-4-RR, Clone: 241871B6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84485-4-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 84485-4-RR (same clone as 84485-4-PBS)

    FC experiment of HeLa using 84485-4-RR (same clone as 84485-4-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug CKAP4 Recombinant antibody (84485-4-RR, Clone:241871B6) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84485-4-PBS in a different storage buffer formulation.

  • FC experiment of U2OS using 84485-4-RR (same clone as 84485-4-PBS)

    FC experiment of U2OS using 84485-4-RR (same clone as 84485-4-PBS)

    1x10^6 U2OS cells were intracellularly stained with 0.25 ug CKAP4 Recombinant antibody (84485-4-RR, Clone:241871B6) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84485-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84485-4-RR

    Affinity and Kinetic Characterization of 84485-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84485-4-RR against Human CKAP4 were performed. The affinity constant is 2.71 nM.

CKAP4 Recombinant antibody, PBS Only

CKAP4 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 84485-4-PBS
CloneNo. 241871B6

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

p63, ERGIC 63, Climp-63, CLIMP 63, 63 kDa membrane protein

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy CKAP4 Ready-To-Use IHC Kit. CKAP4 primary antibody included.

产品信息

84485-4-PBS targets CKAP4 in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, FC (Intra), Indirect ELISA Application Description
Tested Reactivity human
Immunogen CKAP4 fusion protein Ag10022 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name cytoskeleton-associated protein 4
Synonyms p63, ERGIC 63, Climp-63, CLIMP 63, 63 kDa membrane protein
Calculated Molecular Weight 602 aa, 66 kDa
Observed Molecular Weight 63 kDa
GenBank Accession NumberBC082972
Gene Symbol CKAP4
Gene ID (NCBI) 10970
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ07065
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CKAP4 (Cytoskeleton-associated protein 4), also known as CLIMP-63 (cytoskeleton-linking membrane protein 63), is a 63 kDa cytoskeleton-linking membrane protein. CKAP4 is a novel DKK1-binding protein on the cell surface membrane of Madin-Darby canine kidney (MDCK) epithelial cells, and evaluated CKAP4-mediated DKK1 signaling in cancer cell proliferation (PMID: 27322059). In addition, CKAP4 was modified with palmitate at Cys100 by palmitoyl acyltransferase DHHC2 (PMID: 18296695), and palmitoylation was required for CKAP4 localization to PM lipid rafts to promote cancer cell proliferation (PMID: 31744930).