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验证数据展示

  • WB analysis using 83522-5-RR (same clone as 83522-5-PBS)

    WB analysis using 83522-5-RR (same clone as 83522-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83522-5-RR (CKB-Specific antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83522-5-PBS in a different storage buffer formulation.

  • WB analysis of HAP1 using 83522-5-RR (same clone as 83522-5-PBS)

    WB analysis of HAP1 using 83522-5-RR (same clone as 83522-5-PBS)

    WB result of sg-CKB-Specific antibody (83522-5-RR; 1:10000; room temperature for 1.5 hours) with wild-type and sg-CKB-Specific transfected HAP1 cells. This data was developed using the same antibody clone with 83522-5-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 83522-5-RR (same clone as 83522-5-PBS)

    IHC staining of human colon cancer using 83522-5-RR (same clone as 83522-5-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 83522-5-RR (CKB-Specific antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83522-5-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CKB antibody included
  • FC experiment of HeLa using 83522-5-RR (same clone as 83522-5-PBS)

    FC experiment of HeLa using 83522-5-RR (same clone as 83522-5-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug CKB-Specific Recombinant Antibody (83522-5-RR, Clone:240418D2) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 83522-5-PBS in a different storage buffer formulation.

  • Sandwich ELISA sample test of MP00504-4

    Sandwich ELISA sample test of MP00504-4

    The mean CKB concentration was determined to be 138.08 ng/mL in MCF-7 cell extract based on a 1.20 mg/mL extract load, 159.52 ng/mL in HeLa cell extract based on a 1.30 mg/mL extract load, 161.58 ng/mL in mouse brain tissue extract based on a 6.60 mg/mL extract load and 200.17 ng/mL in rat brain tissue extract based on a 8.20 mg/mL extract load.

  • Sandwich ELISA standard curve of MP00504-4

    Sandwich ELISA standard curve of MP00504-4

    Sandwich ELISA standard curve of MP00504-4, Human CKB Recombinant Matched Antibody Pair - PBS only. 83522-5-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag7285. 83522-6-PBS was HRP conjugated as the detection antibody. Range: 1.56-50 ng/mL.

  • Affinity and Kinetic Characterization of 83522-5-PBS

    Affinity and Kinetic Characterization of 83522-5-PBS

    Biolayer interferometry (BLl) kinetic assay of 83522-5-PBS against Human CKB-Specific was performed. The affinity constant is 1.54 nM.

  • Affinity and Kinetic Characterization of 83522-5-RR

    Affinity and Kinetic Characterization of 83522-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83522-5-RR against Human CKB were performed. The affinity constant is below 1 pM.

  • Featured Product
  • KD/KO验证

CKB-Specific Recombinant monoclonal antibody, PBS Only (Capture)

CKB-Specific Uni-rAb® Recombinant Antibody for WB, IHC, FC (Intra), Sandwich ELISA, Indirect ELISA
Cat No. 83522-5-PBS

产品说明书

CloneNo. 240418D2

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, FC (Intra), Sandwich ELISA, Indirect ELISA

别名

CKB, KCRB, 240418D2, B CK, B-CK

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy CKB Ready-To-Use IHC Kit. CKB primary antibody included.

产品信息

83522-5-PBS targets CKB-Specific as part of a matched antibody pair:

MP00504-4: 83522-5-PBS capture and 83522-6-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, FC (Intra), Sandwich ELISA, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag7285

Product name: Recombinant human CKB protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-381 aa of BC001190

Sequence: MPFSNSHNALKLRFPAEDEFPDLSAHNNHMAKVLTPELYAELRAKSTPSGFTLDDVIQTGVDNPGHPYIMTVGCVAGDEESYEVFKDLFDPIIEDRHGGYKPSDEHKTDLNPDNLQGGDDLDPNYVLSSRVRTGRSIRGFCLPPHCSRGERRAIEKLAVEALSSLDGDLAGRYYALKSMTEAEQQQLIDDHFLFDKPVSPLLLASGMARDWPDARGIWHNDNKTFLVWVNEEDHLRVISMQKGGNMKEVFTRFCTGLTQIETLFKSKDYEFMWNPHLGYILTCPSNLGTGLRAGVHIKLPNLGKHEKFSEVLKRLRLQKRGTGGVDTAAVGGVFDVSNADRLGFSEVELVQMVVDGVKLLIEMEQRLEQGQAIDDLMPAQK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 creatine kinase, brain
别名 CKB, KCRB, 240418D2, B CK, B-CK
计算分子量 43 kDa
观测分子量 40-45 kDa
GenBank蛋白编号BC001190
基因名称 CKB
Gene ID (NCBI) 1152
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDP12277
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CKBB, also named as B-CK and CKB, is a member of the ATP:guanido phosphotransferase protein family. It is a cytoplasmic enzyme involved in energy homeostasis. CKBB reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in brain as well as in other tissues, and as a heterodimer with a similar muscle isozyme in heart. CK isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa. CK MB consists of a dimer of nonidentical chains. With MM being the major form in skeletal muscle and myocardium, MB existing in myocardium, and BB existing in many tissues, especially brain.

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