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CKB Recombinant antibody

CKB Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240418D2

Cat no : 83522-5-RR

Print datasheet

Synonyms

KCRB, Creatine kinase B-type, CPK-B, CKBB, B-CK

验证数据展示

  • WB analysis using 83522-5-RR

    WB analysis using 83522-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83522-5-RR (CKB antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • FC experiment of HeLa using 83522-5-RR

    FC experiment of HeLa using 83522-5-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug CKB Recombinant Antibody (83522-5-RR, Clone:240418D2) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inHeLa cells, SH-SY5Y cells, Y79 cells, mouse brain tissue, rat brain tissue
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy CKB Ready-To-Use IHC Kit. CKB primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83522-5-RR targets CKB in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, FC (Intra), ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen CKB fusion protein Ag7285 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name creatine kinase, brain
Synonyms KCRB, Creatine kinase B-type, CPK-B, CKBB, B-CK
Calculated Molecular Weight 43 kDa
Observed Molecular Weight 40-45 kDa
GenBank Accession NumberBC001190
Gene Symbol CKB
Gene ID (NCBI) 1152
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP12277
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CKBB, also named as B-CK and CKB, is a member of the ATP:guanido phosphotransferase protein family. It is a cytoplasmic enzyme involved in energy homeostasis. CKBB reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in brain as well as in other tissues, and as a heterodimer with a similar muscle isozyme in heart. CK isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa. CK MB consists of a dimer of nonidentical chains. With MM being the major form in skeletal muscle and myocardium, MB existing in myocardium, and BB existing in many tissues, especially brain. This antibody can recognize both CKB and CKM due to the high homology.

实验方案

Product Specific Protocols
WB protocol for CKB antibody 83522-5-RRDownload protocol
FC protocol for CKB antibody 83522-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols