验证数据展示
经过测试的应用
| Positive WB detected in | Human skeletal muscle, human skeletal muscle tissue |
| Positive IHC detected in | human liver tissue, mouse heart tissue, mouse testis tissue, rat brain tissue, rat testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse heart tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
60177-1-Ig targets CKM-Specific in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF-P, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2a |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | creatine kinase, muscle |
| 别名 | CKM, 2G3F6, Creatine kinase M-type, CPK-M, CKMM |
| 计算分子量 | 43 kDa |
| 观测分子量 | 43 kDa, 90 kDa, 130 kDa |
| GenBank蛋白编号 | BC007462 |
| 基因名称 | CKM |
| Gene ID (NCBI) | 1158 |
| RRID | AB_10897180 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P06732 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CKM, also named as CKMM and M-CK, is a member of the ATP:guanido phosphotransferase protein family. It is a cytoplasmic enzyme involved in energy homeostasis and is an important serum marker for myocardial infarction. CKM reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in striated muscle as well as in other tissues, and as a heterodimer with a similar brain isozyme in heart. CK isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa. CK MB consists of a dimer of nonidentical chains. With MM being the major form in skeletal muscle and myocardium, MB existing in myocardium. CKM has a calculated molecular mass of 43 kDa, and the 90-kDa and 130-kDa bands could be due to acovalent cross-linking of two and three CKm subunits, respectively (PMID: 20195383).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CKM-Specific antibody 60177-1-Ig | Download protocol |
| IHC protocol for CKM-Specific antibody 60177-1-Ig | Download protocol |
| IF protocol for CKM-Specific antibody 60177-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
