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CoraLite® Plus 488-conjugated CKM-Specific Monoclonal antibody

CKM-Specific Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2G3F6

Cat no : CL488-60177

Print datasheet

Synonyms

CKM, CKMM, CKM-Specific, Creatine kinase M chain, Creatine kinase M type, creatine kinase, muscle, M CK

验证数据展示

  • IF Staining of C2C12 using CL488-60177

    IF Staining of C2C12 using CL488-60177

    Immunofluorescent analysis of (-20°C Ethanol) fixed C2C12 cells using CL488-60177 (CKM-Specific antibody) at dilution of 1:100.

经过测试的应用

Positive IF/ICC detected inC2C12 cells
For other applications, we recommend the unconjugated version of this antibody, 60177-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-60177 targets CKM-Specific in IF/ICC applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human, mouse, rat
Immunogen Peptide 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name creatine kinase, muscle
Synonyms CKM, CKMM, CKM-Specific, Creatine kinase M chain, Creatine kinase M type, creatine kinase, muscle, M CK
Calculated Molecular Weight 43 kDa
Observed Molecular Weight 43 kDa
GenBank Accession NumberBC007462
Gene Symbol CKM
Gene ID (NCBI) 1158
RRIDAB_2883110
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP06732
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CKM, also named as CKMM and M-CK, is a member of the ATP:guanido phosphotransferase protein family. It is a cytoplasmic enzyme involved in energy homeostasis and is an important serum marker for myocardial infarction. CKM reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in striated muscle as well as in other tissues, and as a heterodimer with a similar brain isozyme in heart. CK isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa. CK MB consists of a dimer of nonidentical chains. With MM being the major form in skeletal muscle and myocardium, MB existing in myocardium, and BB existing in many tissues, especially brain.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 CKM-Specific antibody CL488-60177Download protocol
Standard Protocols
Click here to view our Standard Protocols