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验证数据展示

  • WB analysis using 68853-7-Ig

    WB analysis using 68853-7-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68853-7-Ig (CLCC1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • WB analysis using 68853-7-Ig

    WB analysis using 68853-7-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68853-7-Ig (CLCC1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

CLCC1 Monoclonal antibody

CLCC1 Monoclonal Antibody for WB, ELISA
Cat No. 68853-7-Ig

产品说明书

CloneNo. 3C4B4

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, ELISA

别名

Mid-1-related chloride channel protein 1, MCLC, KIAA0761, Chloride channel CLIC-like protein 1, chloride channel CLIC like 1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inCOLO 320 cells, HepG2 cells, hTERT-RPE1 cells, HeLa cells, K-562 cells, FaDu cells, SiHa cells, SCaBER cells, MG-63 cells, MDA-MB-468 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68853-7-Ig targets CLCC1 in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原 CLCC1 fusion protein Ag24682 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 chloride channel CLIC-like 1
别名 Mid-1-related chloride channel protein 1, MCLC, KIAA0761, Chloride channel CLIC-like protein 1, chloride channel CLIC like 1
计算分子量 551 aa, 62 kDa
观测分子量 50-60 kDa
GenBank蛋白编号BC002939
基因名称 CLCC1
Gene ID (NCBI) 23155
RRIDAB_3670441
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ96S66
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CLCC1, also known as MCLC, is a transmembrane protein that localizes to intracellular compartments, including endoplasmic reticulum (ER), and it functions as a chloride channel (PMID: 11279057). It has been shown that loss of CLCC1 results in ER Stress, misfolded protein accumulation, and neurodegeneration (PMID: 25698737). CLCC1 forms homomultimer in the ER membrane and is a pore-forming component of an anion channel. Studies found that disruption of ER ion homeostasis maintained by CLCC1 is one of the causes of neurodegenerative diseases, such as ALS (amyotrophic lateral sclerosis) (PMID: 37142673).

实验方案

Product Specific Protocols
WB protocol for CLCC1 antibody 68853-7-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols