Claudin 18 Monoclonal antibody
Claudin 18 Monoclonal Antibody for IP, IHC, ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
human, mouse
Applications
IP, IHC, ELISA and More (1)
Conjugate
Unconjugated
CloneNo.
5G7F2
验证数据展示
经过测试的应用
Positive IP detected in | mouse stomach tissue |
Positive IHC detected in | human stomach tissue, human pancreas cancer tissue, human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
Application | Dilution |
---|---|
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
IHC | See 1 publications below |
IF | See 2 publications below |
产品信息
66167-1-Ig targets Claudin 18 in IP, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.
Tested Applications | IP, IHC, ELISA Application Description |
Cited Applications | IF, IHC |
Tested Reactivity | human, mouse |
Cited Reactivity | human, mouse |
Immunogen | Claudin 18 fusion protein Ag15517 种属同源性预测 |
Host / Isotype | Mouse / IgG2a |
Class | Monoclonal |
Type | Antibody |
Full Name | claudin 18 |
Synonyms | claudin 18, CLDN18, SFTA5, SFTPJ |
Calculated Molecular Weight | 261 aa, 28 kDa |
Observed Molecular Weight | 29 kDa |
GenBank Accession Number | BC146668 |
Gene Symbol | CLDN18 |
Gene ID (NCBI) | 51208 |
RRID | AB_2881563 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P56856 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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IHC protocol for Claudin 18 antibody 66167-1-Ig | Download protocol |
IP protocol for Claudin 18 antibody 66167-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Mucosal Immunol Airway epithelium IgE-FcεRI Cross-Link Induces Epithelial Barrier Disruption in Severe T2-high Asthma | ||
World J Gastrointest Pathophysiol Visualization of sphingolipids and phospholipids in the fundic gland mucosa of human stomach using imaging mass spectrometry. |