验证数据展示
经过测试的应用
| Positive WB detected in | K-562 cells, Raji cells, mouse testis tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
84748-1-RR targets CLEC10A/CD301 in WB, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse |
| 免疫原 | Recombinant protein 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | C-type lectin domain family 10, member A |
| 别名 | CLEC10A, C-type lectin domain family 10 member A, CLECSF14, CLECSF13, CD301 |
| 计算分子量 | 316 aa, 35 kDa |
| 观测分子量 | 35 kDa |
| GenBank蛋白编号 | BC039011 |
| 基因名称 | CLEC10A |
| Gene ID (NCBI) | 10462 |
| RRID | AB_3672131 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purfication |
| UNIPROT ID | Q8IUN9 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
C-type lectin domain family 10 member A (CLEC10A), also known as CD301, macrophage galactose-type C-type lectin (MGL), DC-asialoglyco protein receptor (DC-ASGP-R) or human macrophage lectin (HML), is a type II transmembrane glycoprotein having a calcium-dependent carbohydrate recognition domain (PMID: 8598452; 11919201; 11698450). CLEC10A is expressed exclusively by myeloid professional antigen-presenting cells such as immature dendritic cells (DCs) and alternatively activated macrophages (PMID: 16998493). CLEC10A is a marker for human CD1c+ DCs (cDC2) (PMID: 29755453). It functions as an endocytic receptor for glycosylated antigens (PMID: 11919201).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CLEC10A/CD301 antibody 84748-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |