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验证数据展示

  • FC experiment of human peripheral blood leukocytes using 98083-1-RR

    FC experiment of human peripheral blood leukocytes using 98083-1-RR

    1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human CLEC12A Rabbit Recombinant Antibody (98083-1-RR, Clone:240791B7), or Isotype Control and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with CoraLite® Plus 647 Anti-Human CD16. Cells were incubated with FC Receptor Block prior to fixing and staining. Cells were not fixed.

  • FC experiment of human peripheral blood leukocytes using 98083-1-RR

    FC experiment of human peripheral blood leukocytes using 98083-1-RR

    1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human CLEC12A Rabbit Recombinant Antibody (98083-1-RR, Clone:240791B7)(red), or 0.25 ug Isotype Control(blue) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to fixing and staining.Cells were not fixed.

  • Affinity and Kinetic Characterization of 98083-1-RR

    Affinity and Kinetic Characterization of 98083-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98083-1-RR against Human CLEC12A were performed. The affinity constant is 0.524 nM.

Anti-Human CLEC12A Rabbit Recombinant Antibody

CLEC12A Uni-rAbTM Recombinant Antibody for FC
Cat No. 98083-1-RR

产品说明书

CloneNo. 240791B7

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

FC

别名

CLL-1, CLL1, CLL 1, CD371, C type lectin like molecule 1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive FC detected inhuman peripheral blood leukocytes
Planning an IHC experiment? We recommend our IHCeasy CLEC12A Ready-To-Use IHC Kit. CLEC12A primary antibody included.

推荐稀释比

应用推荐稀释比
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

98083-1-RR targets CLEC12A in FC applications and shows reactivity with human samples.

经测试应用 FC Application Description
经测试反应性 human
免疫原 Fusion Protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 C-type lectin domain family 12, member A
别名 CLL-1, CLL1, CLL 1, CD371, C type lectin like molecule 1
计算分子量31 kDa
GenBank蛋白编号BC126291
基因名称 CLEC12A
Gene ID (NCBI) 160364
RRIDAB_3672230
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ5QGZ9
储存缓冲液 PBS with 0.09% sodium azide , pH 7.3
储存条件Store at 2 - 8°C. Stable for one year after shipment.

背景介绍

CLEC12A, also known as CD371, MICL, DCAL-2 or CLL-1, is a type II transmembrane glycoprotein and a member of the C-type lectin superfamily (PMID: 30401586). This protein, which contains a single C-type lectin-like domain and a cytoplasmic immunoreceptor tyrosine-based inhibitory motif (ITIM), is related to LOX-1 and Dectin-1 and is variably spliced and highly N-glycosylated (PMID: 14739280). Its ITIM sequence recruits the tyrosine phosphatases SHP-1 and SHP-2 to negatively regulate Syk signaling. In humans, CLEC12A is mainly expressed on myeloid cells, being found on monocytes, granulocytes, macrophages and DC subsets, and at lower levels on CD4+ T cells (PMID: 32477350). The observed molecular weight of CLEC12A is between 40 and 75 kDa, which is much higher than the predicted molecular weight due to glycosylation (PMID: 14739280).

实验方案

Product Specific Protocols
FC protocol for CLEC12A antibody 98083-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols