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CLEC14A Polyclonal antibody

CLEC14A Polyclonal Antibody for FC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC, WB, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 23964-1-AP

Print datasheet

Synonyms

C14orf27, CEG1, CLEC14A

验证数据展示

  • FC experiment of HUVEC using 23964-1-AP

    FC experiment of HUVEC using 23964-1-AP

    1X10^6 HUVEC cells were stained with 0.2ug CLEC14A antibody (23964-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

  • WB analysis using 23964-1-AP

    WB analysis using 23964-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 23964-1-AP (CLEC14A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inmouse brain tissue, mouse skeletal muscle tissue, human heart tissue, rat brain tissue
Positive FC detected inHUVEC cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

23964-1-AP targets CLEC14A in WB, IHC, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC, WB, ELISA
Cited ApplicationsIHC, WB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen CLEC14A fusion protein Ag21081 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name C-type lectin domain family 14, member A
Synonyms C14orf27, CEG1, CLEC14A
Calculated Molecular Weight 490 aa, 52 kDa
Observed Molecular Weight 52 kDa
GenBank Accession NumberBC031567
Gene Symbol CLEC14A
Gene ID (NCBI) 161198
RRIDAB_2879377
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
UNIPROT IDQ86T13
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CLEC14A is a single-pass type I transmembrane protein whose extracellular domain consists of a C-type lectin-like domain (CTLD), a series of EGF-like domains, and a sushi-like domain (PMID: 23644659). CLEC14A is a member of the C-type lectin domain superfamily that also includes endosialin, thrombomodulin and CD93 (PMID: 16336259). It has been reported that CLEC14A is specifically expressed in endothelial cells and mediates cell to cell adhesion (PMID: 21095181). CLEC14A may be involved in tumor angiogenesis (PMID: 21706054; 23644659).

实验方案

Product Specific Protocols
WB protocol for CLEC14A antibody 23964-1-APDownload protocol
FC protocol for CLEC14A antibody 23964-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,mouseWB,IHC

FASEB J

CLEC14A protects against podocyte injury in mice with adriamycin nephropathy.

Authors - Zeyu Su
View Article