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验证数据展示

  • WB analysis of JAR using 84759-4-RR

    WB analysis of JAR using 84759-4-RR

    JAR cells were subjected to SDS PAGE followed by western blot with 84759-4-RR (CLIC3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of A431 using 84759-4-RR

    IF Staining of A431 using 84759-4-RR

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using CLIC3 antibody (84759-4-RR, Clone: 242326H7 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 84759-4-RR

    Affinity and Kinetic Characterization of 84759-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84759-4-RR against Human CLIC3 were performed. The affinity constant is 0.634 nM.

CLIC3 Recombinant antibody

CLIC3 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 84759-4-RR

Print datasheet

CloneNo. 242326H7

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

242326H7

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inJAR cells, HeLa cells
Positive IF/ICC detected inA431 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84759-4-RR targets CLIC3 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen CLIC3 fusion protein Ag8757 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name chloride intracellular channel 3
Synonyms 242326H7
Calculated Molecular Weight 236 aa, 26 kDa
Observed Molecular Weight 27-32 kDa
GenBank Accession NumberBC007012
Gene Symbol CLIC3
Gene ID (NCBI) 9022
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDO95833
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Chloride channels are a diverse group of proteins that regulate fundamental cellular processes including stabilization of cell membrane potential, transepithelial transport, maintenance of intracellular pH, and regulation of cell volume (PMID: 9880541). CLIC3 (chloride intracellular channel protein 3) belongs to the chloride channel CLIC family which has seven members based on sequence homology to the p64 chloride channels of bovine kidney microsomes. CLIC proteins lack the membrane-spanning domains characteristic of channel proteins and are largely intracellular. CLIC3 is predominantly localized in the nucleus and has a high expression level in placenta (PMID: 9880541; 17027078). It associates with the C-terminal of ERK7 and may participate in cellular growth control.

实验方案

Product Specific Protocols
WB protocol for CLIC3 antibody 84759-4-RRDownload protocol
IF protocol for CLIC3 antibody 84759-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols