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验证数据展示

  • WB analysis of HeLa using 66343-1-Ig (same clone as 66343-1-PBS)

    WB analysis of HeLa using 66343-1-Ig (same clone as 66343-1-PBS)

    WB result of CLIC4 antibody (66343-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CLIC4 transfected HeLa cells. This data was developed using the same antibody clone with 66343-1-PBS in a different storage buffer formulation.

  • WB analysis using 66343-1-Ig (same clone as 66343-1-PBS)

    WB analysis using 66343-1-Ig (same clone as 66343-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66343-1-Ig (CLIC4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66343-1-PBS in a different storage buffer formulation.

  • IHC staining of human heart using 66343-1-Ig (same clone as 66343-1-PBS)

    IHC staining of human heart using 66343-1-Ig (same clone as 66343-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66343-1-Ig (CLIC4 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66343-1-PBS in a different storage buffer formulation.

  • IHC staining of human heart using 66343-1-Ig (same clone as 66343-1-PBS)

    IHC staining of human heart using 66343-1-Ig (same clone as 66343-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66343-1-Ig (CLIC4 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66343-1-PBS in a different storage buffer formulation.

  • FC experiment of BT-549 using 66343-1-Ig (same clone as 66343-1-PBS)

    FC experiment of BT-549 using 66343-1-Ig (same clone as 66343-1-PBS)

    1x10^6 BT-549 cells were intracellularly stained with 0.2 μg CLIC4 Monoclonal antibody (66343-1-Ig, Clone: 1B7F9) and CoraLite® Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (H+L)(Cat.NO.RGAM005). Mouse IgG1 isotype control (66360-1-Ig, Clone: 1F8D3, blue) was parallel stained as control. Cells were fixed with 4% PFA. This data was developed using the same antibody clone with 66343-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 66343-1-Ig (same clone as 66343-1-PBS)

    IF Staining of HeLa using 66343-1-Ig (same clone as 66343-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CLIC4 antibody (66343-1-Ig, Clone: 1B7F9 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66343-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

CLIC4 Monoclonal antibody, PBS Only

CLIC4 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 66343-1-PBS

产品说明书

CloneNo. 1B7F9

宿主/亚型

Mouse / IgG1

种属反应性

human, rat, pig

应用

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

别名

MTCLIC, huH1, H1, FLJ38640, CLIC4L

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

66343-1-PBS targets CLIC4 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, rat, pig samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, rat, pig
免疫原 CLIC4 fusion protein Ag2942 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 chloride intracellular channel 4
别名 MTCLIC, huH1, H1, FLJ38640, CLIC4L
计算分子量 253 aa, 29 kDa
观测分子量 29 kDa
GenBank蛋白编号BC012444
基因名称 CLIC4
Gene ID (NCBI) 25932
RRIDAB_2881723
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ9Y696
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Chloride channels are a diverse group of proteins that regulate fundamental cellular processes including stabilization of cell membrane potential, transepithelial transport, maintenance of intracellular pH, and regulation of cell volume. CLIC4 is a p53- and tumor necrosis factor alpha-regulated cytoplasmic and mitochondrial protein that belongs to the CLIC family of intracellular chloride channels. CLICs have actions distinct from traditional cell membrane chloride channels, including formation of ion channels in intracellular organelles and roles in membrane trafficking, apoptosis, and cell differentiation.