WB result of CLPX antibody (83097-1-RR; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CLPX transfected A549 cells. This data was developed using the same antibody clone with 83097-1-PBS in a different storage buffer formulation.

Various lysates were subjected to SDS PAGE followed by western blot with 83097-1-RR (CLPX antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83097-1-PBS in a different storage buffer formulation.

1x10^6 HeLa cells were intracellularly stained with 0.25 ug Anti-Human CLPX (83097-1-RR, Clone:230346H1) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 83097-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 83097-1-RR (CLPX antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83097-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 83097-1-RR (CLPX antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83097-1-PBS in a different storage buffer formulation.

The mean CLPX concentration was determined to be 163.0 ng/mL in HeLa cell extract based on a 1.2 mg/mL extract load and 80.9 ng/mL in A549 cell extract based on a 1.0 mg/mL extract load.

Sandwich ELISA standard curve of MP00277-3, Human CLPX Recombinant Matched Antibody Pair - PBS only. 83097-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag34213. 83097-1-PBS was HRP conjugated as the detection antibody. Range: 0.78-50 ng/mL.

Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using CLPX antibody (83097-1-RR, Clone: 230346H1 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83097-1-PBS in a different storage buffer formulation.

Biolayer interferometry (BLl) kinetic assays of 83097-1-RR against Human CLPX were performed. The affinity constant is 2.13 nM.