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验证数据展示

  • WB analysis of A549 using 83097-1-RR (same clone as 83097-1-PBS)

    WB analysis of A549 using 83097-1-RR (same clone as 83097-1-PBS)

    WB result of CLPX antibody (83097-1-RR; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CLPX transfected A549 cells. This data was developed using the same antibody clone with 83097-1-PBS in a different storage buffer formulation.

  • WB analysis using 83097-1-RR (same clone as 83097-1-PBS)

    WB analysis using 83097-1-RR (same clone as 83097-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83097-1-RR (CLPX antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83097-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 83097-1-RR (same clone as 83097-1-PBS)

    FC experiment of HeLa using 83097-1-RR (same clone as 83097-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug Anti-Human CLPX (83097-1-RR, Clone:230346H1) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83097-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 83097-1-RR (same clone as 83097-1-PBS)

    IHC staining of human liver using 83097-1-RR (same clone as 83097-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 83097-1-RR (CLPX antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83097-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 83097-1-RR (same clone as 83097-1-PBS)

    IHC staining of human liver using 83097-1-RR (same clone as 83097-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 83097-1-RR (CLPX antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83097-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP00277-3

    Sandwich ELISA standard curve of MP00277-3

    Sandwich ELISA standard curve of MP00277-3, Human CLPX Recombinant Matched Antibody Pair - PBS only. 83097-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag34213. 83097-1-PBS was HRP conjugated as the detection antibody. Range: 0.78-50 ng/mL.

  • Sample test of MP00277-3

    Sample test of MP00277-3

    The mean CLPX concentration was determined to be 163.0 ng/mL in HeLa cell extract based on a 1.2 mg/mL extract load and 80.9 ng/mL in A549 cell extract based on a 1.0 mg/mL extract load.

  • Cytometric bead array standard curve of MP00277-1

    Cytometric bead array standard curve of MP00277-1

    Cytometric bead array standard curve of MP00277-1, CLPX Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83097-1-PBS. Detection antibody: 83097-2-PBS. Standard: Ag34213. Range: 0.625-20 ng/mL.

  • Affinity and Kinetic Characterization of 83097-1-RR

    Affinity and Kinetic Characterization of 83097-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83097-1-RR against Human CLPX were performed. The affinity constant is 2.13 nM.

  • Featured Product
  • KD/KO Validated

CLPX Recombinant antibody, PBS Only (Capture/Detector)

CLPX Uni-rAbTM Recombinant Antibody for WB, IHC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 83097-1-PBS

Print datasheet

CloneNo. 230346H1

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

EPP2, 230346H1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size: 

-/ -

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产品信息

83097-1-PBS targets CLPX as part of a matched antibody pair:

MP00277-1: 83097-1-PBS capture and 83097-2-PBS detection (validated in Cytometric bead array)

MP00277-3: 83097-2-PBS capture and 83097-1-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, IHC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
Tested Reactivity human, mouse, rat
Immunogen CLPX fusion protein Ag34213 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name ClpX caseinolytic peptidase X homolog (E. coli)
Synonyms EPP2, 230346H1
Calculated Molecular Weight69 kDa
Observed Molecular Weight63 kDa
GenBank Accession NumberNM_006660
Gene Symbol CLPX
Gene ID (NCBI) 10845
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO76031
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C