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验证数据展示

  • WB analysis of A549 using 83097-1-RR

    WB analysis of A549 using 83097-1-RR

    WB result of CLPX antibody (83097-1-RR; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CLPX transfected A549 cells.

  • WB analysis using 83097-1-RR

    WB analysis using 83097-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83097-1-RR (CLPX antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • FC experiment of HeLa using 83097-1-RR

    FC experiment of HeLa using 83097-1-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug Anti-Human CLPX (83097-1-RR, Clone:230346H1) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IHC staining of human liver using 83097-1-RR

    IHC staining of human liver using 83097-1-RR

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 83097-1-RR (CLPX antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver using 83097-1-RR

    IHC staining of human liver using 83097-1-RR

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 83097-1-RR (CLPX antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 83097-1-RR

    Affinity and Kinetic Characterization of 83097-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83097-1-RR against Human CLPX were performed. The affinity constant is 2.13 nM.

  • Featured Product
  • KD/KO验证

CLPX Recombinant antibody

CLPX Uni-rAbTM Recombinant Antibody for WB, IHC, FC (Intra), ELISA
Cat No. 83097-1-RR

产品说明书

CloneNo. 230346H1

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, FC (Intra), ELISA

别名

EPP2, 230346H1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHepG2 cells, A549 cells, HeLa cells, K-652 cells, HT-1080 cells, Saos-2 cells
Positive IHC detected inhuman liver tissue, mouse stomach tissue, rat testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83097-1-RR targets CLPX in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, FC (Intra), ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 CLPX fusion protein Ag34213 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ClpX caseinolytic peptidase X homolog (E. coli)
别名 EPP2, 230346H1
计算分子量69 kDa
观测分子量63 kDa
GenBank蛋白编号NM_006660
基因名称 CLPX
Gene ID (NCBI) 10845
RRIDAB_3670811
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO76031
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CLPX antibody 83097-1-RRDownload protocol
IHC protocol for CLPX antibody 83097-1-RRDownload protocol
FC protocol for CLPX antibody 83097-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols