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验证数据展示

  • WB analysis of A549 using 83097-1-RR

    WB analysis of A549 using 83097-1-RR

    WB result of CLPX antibody (83097-1-RR; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CLPX transfected A549 cells.

  • WB analysis using 83097-1-RR

    WB analysis using 83097-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83097-1-RR (CLPX antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • FC experiment of HeLa using 83097-1-RR

    FC experiment of HeLa using 83097-1-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug Anti-Human CLPX (83097-1-RR, Clone:230346H1) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IHC staining of human liver using 83097-1-RR

    IHC staining of human liver using 83097-1-RR

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 83097-1-RR (CLPX antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver using 83097-1-RR

    IHC staining of human liver using 83097-1-RR

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 83097-1-RR (CLPX antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 83097-1-RR

    Affinity and Kinetic Characterization of 83097-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83097-1-RR against Human CLPX were performed. The affinity constant is 2.13 nM.

  • Featured Product
  • KD/KO Validated

CLPX Recombinant antibody

CLPX Uni-rAbTM Recombinant Antibody for WB, IHC, FC (Intra), ELISA
Cat No. 83097-1-RR

Print datasheet

CloneNo. 230346H1

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, FC (Intra), ELISA

Synonyms

EPP2, 230346H1

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inHepG2 cells, A549 cells, HeLa cells, K-652 cells, HT-1080 cells, Saos-2 cells
Positive IHC detected inhuman liver tissue, mouse stomach tissue, rat testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83097-1-RR targets CLPX in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, FC (Intra), ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen CLPX fusion protein Ag34213 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name ClpX caseinolytic peptidase X homolog (E. coli)
Synonyms EPP2, 230346H1
Calculated Molecular Weight69 kDa
Observed Molecular Weight63 kDa
GenBank Accession NumberNM_006660
Gene Symbol CLPX
Gene ID (NCBI) 10845
RRIDAB_3670811
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO76031
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CLPX antibody 83097-1-RRDownload protocol
IHC protocol for CLPX antibody 83097-1-RRDownload protocol
FC protocol for CLPX antibody 83097-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols