验证数据展示
经过测试的应用
| Positive WB detected in | pig brain tissue |
| Positive IHC detected in | human liver tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy CLTB Ready-To-Use IHC Kit. CLTB primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
66270-1-Ig targets CLTB in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, pig samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, pig |
| 文献引用反应性 | human |
| 免疫原 | CLTB fusion protein Ag24347 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | clathrin, light chain (Lcb) |
| 别名 | Clathrin light chain B, clathrin, light chain (Lcb), CLTB, LCB |
| 计算分子量 | 23 kDa |
| 观测分子量 | 32-38 kDa |
| GenBank蛋白编号 | BC006457 |
| 基因名称 | CLTB |
| Gene ID (NCBI) | 1212 |
| RRID | AB_2881655 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | P09497 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Clathrin is the major protein of the polyhedral coat of coated pits and vesicles which entrap specific macromolecules during receptor-mediated endocytosis. The clathrin molecule has a triskelion shape. Each clathrin triskelion is composed of three identical heavy chains (180 kDa) and three light chains of two types, LCA (CLTA) and LCB (CLTB) (30-40 kDa). The light chain subunits are thought to regulate the formation or disassembly of clathrin coats. (PMID: 2445759; 8374173; 3563513)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CLTB antibody 66270-1-Ig | Download protocol |
| IHC protocol for CLTB antibody 66270-1-Ig | Download protocol |
| IF protocol for CLTB antibody 66270-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
