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CLTB Monoclonal antibody, PBS Only

CLTB Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, pig

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1F9C2

Cat no : 66270-1-PBS

Print datasheet

Synonyms

Clathrin light chain B, clathrin, light chain (Lcb), CLTB, LCB

验证数据展示

  • WB analysis of pig brain using 66270-1-Ig (same clone as 66270-1-PBS)

    WB analysis of pig brain using 66270-1-Ig (same clone as 66270-1-PBS)

    pig brain tissue were subjected to SDS PAGE followed by western blot with 66270-1-Ig (CLTB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66270-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 66270-1-Ig (same clone as 66270-1-PBS)

    IHC staining of human liver using 66270-1-Ig (same clone as 66270-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66270-1-Ig (CLTB Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66270-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CLTB antibody included
  • IHC staining of human liver using 66270-1-Ig (same clone as 66270-1-PBS)

    IHC staining of human liver using 66270-1-Ig (same clone as 66270-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66270-1-Ig (CLTB Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66270-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CLTB antibody included
  • IF Staining of HeLa using 66270-1-Ig (same clone as 66270-1-PBS)

    IF Staining of HeLa using 66270-1-Ig (same clone as 66270-1-PBS)

    Immunofluorescent analysis of (-20℃ Ethanol ) fixed HeLa cells using 66270-1-Ig(CLTB antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66270-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy CLTB Ready-To-Use IHC Kit. CLTB primary antibody included.

产品信息

66270-1-PBS targets CLTB in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, pig samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human, pig
Immunogen CLTB fusion protein Ag24347 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name clathrin, light chain (Lcb)
Synonyms Clathrin light chain B, clathrin, light chain (Lcb), CLTB, LCB
Calculated Molecular Weight 23 kDa
Observed Molecular Weight 32-38 kDa
GenBank Accession NumberBC006457
Gene Symbol CLTB
Gene ID (NCBI) 1212
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP09497
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Clathrin is the major protein of the polyhedral coat of coated pits and vesicles which entrap specific macromolecules during receptor-mediated endocytosis. The clathrin molecule has a triskelion shape. Each clathrin triskelion is composed of three identical heavy chains (180 kDa) and three light chains of two types, LCA (CLTA) and LCB (CLTB) (30-40 kDa). The light chain subunits are thought to regulate the formation or disassembly of clathrin coats. (PMID: 2445759; 8374173; 3563513)