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CLTC Monoclonal antibody
CLTC Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse, rat, pig, rabbit and More (1)
Applications
WB, IHC, IF/ICC, ELISA and More (2)
Conjugate
Unconjugated
CloneNo.
1B3D7
验证数据展示
经过测试的应用
| Positive WB detected in | LNCaP cells, HeLa cells, Raji cells, rat brain tissue, Jurkat cells, ROS1728 cells, pig brain tissue, HEK-293 cells, K-562 cells, rabbit brain tissue, mouse brain tissue |
| Positive IHC detected in | mouse kidney tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells, HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy CLTC Ready-To-Use IHC Kit. CLTC primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:20000-1:100000 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
66487-1-Ig targets CLTC in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.
| Tested Applications | WB, IHC, IF/ICC, ELISA Application Description |
| Cited Applications | WB, IF, CoIP |
| Tested Reactivity | human, mouse, rat, pig, rabbit |
| Cited Reactivity | human, mouse, rat, pig, monkey |
| Immunogen | CLTC fusion protein Ag25040 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | clathrin, heavy chain (Hc) |
| Synonyms | clathrin, heavy chain (Hc), Clathrin heavy chain 1, Clathrin heavy chain, CHC17, CHC |
| Calculated Molecular Weight | 192 kDa |
| Observed Molecular Weight | 170 kDa |
| GenBank Accession Number | BC054489 |
| Gene Symbol | CLTC |
| Gene ID (NCBI) | 1213 |
| RRID | AB_2881852 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q00610 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Clathrin is the major protein of the polyhedral coat of coated pits and vesicles which entrap specific macromolecules during receptor-mediated endocytosis. The clathrin molecule has a triskelion shape. Each clathrin triskelion is composed of three identical heavy chains (CLTC) (160-190 kDa) and three light chains of two types, LCA (CLTA) and LCB (CLTB) (30-40 kDa). The heavy chain provides the structural backbone of the clathrin lattice. (PMID: 9133677)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CLTC antibody 66487-1-Ig | Download protocol |
| IHC protocol for CLTC antibody 66487-1-Ig | Download protocol |
| IF protocol for CLTC antibody 66487-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Front Microbiol Porcine Circovirus Type 3 Enters Into PK15 Cells Through Clathrin- and Dynamin-2-Mediated Endocytosis in a Rab5/Rab7 and pH-Dependent Fashion. | ||
Cell Prolif Perturbation of intestinal stem cell homeostasis and radiation enteritis recovery via dietary titanium dioxide nanoparticles | ||
J Virol Porcine Enteric Alphacoronavirus Entry through Multiple Pathways (Caveolae, Clathrin, and Macropinocytosis) Requires Rab GTPases for Endosomal Transport | ||
Neuron Species-specific FMRP regulation of RACK1 is critical for prenatal cortical development |
