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  • Featured Product
  • KD/KO Validated

CLTC Monoclonal antibody, PBS Only

CLTC Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1B3D7

Cat no : 66487-1-PBS

Print datasheet

Synonyms

clathrin, heavy chain (Hc), Clathrin heavy chain 1, Clathrin heavy chain, CHC17, CHC

验证数据展示

  • WB analysis of HeLa using 66487-1-Ig (same clone as 66487-1-PBS)

    WB analysis of HeLa using 66487-1-Ig (same clone as 66487-1-PBS)

    WB result of CLTC antibody (66487-1-Ig; 1:60000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CLTC transfected HeLa cells. This data was developed using the same antibody clone with 66487-1-PBS in a different storage buffer formulation.

  • WB analysis using 66487-1-Ig (same clone as 66487-1-PBS)

    WB analysis using 66487-1-Ig (same clone as 66487-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66487-1-Ig (CLTC antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66487-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse kidney using 66487-1-Ig (same clone as 66487-1-PBS)

    IHC staining of mouse kidney using 66487-1-Ig (same clone as 66487-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 66487-1-Ig (CLTC antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66487-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CLTC antibody included
  • IHC staining of mouse kidney using 66487-1-Ig (same clone as 66487-1-PBS)

    IHC staining of mouse kidney using 66487-1-Ig (same clone as 66487-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 66487-1-Ig (CLTC antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66487-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CLTC antibody included
  • IF Staining of HepG2 using 66487-1-Ig (same clone as 66487-1-PBS)

    IF Staining of HepG2 using 66487-1-Ig (same clone as 66487-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CLTC antibody (66487-1-Ig, Clone: 1B3D7 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66487-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy CLTC Ready-To-Use IHC Kit. CLTC primary antibody included.

产品信息

66487-1-PBS targets CLTC in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat, pig, rabbit
Immunogen CLTC fusion protein Ag25040 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name clathrin, heavy chain (Hc)
Synonyms clathrin, heavy chain (Hc), Clathrin heavy chain 1, Clathrin heavy chain, CHC17, CHC
Calculated Molecular Weight 192 kDa
Observed Molecular Weight 170 kDa
GenBank Accession NumberBC054489
Gene Symbol CLTC
Gene ID (NCBI) 1213
RRIDAB_2881852
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ00610
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Clathrin is the major protein of the polyhedral coat of coated pits and vesicles which entrap specific macromolecules during receptor-mediated endocytosis. The clathrin molecule has a triskelion shape. Each clathrin triskelion is composed of three identical heavy chains (CLTC) (160-190 kDa) and three light chains of two types, LCA (CLTA) and LCB (CLTB) (30-40 kDa). The heavy chain provides the structural backbone of the clathrin lattice. (PMID: 9133677)