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  • Featured Product
  • KD/KO Validated

CLUAP1 Polyclonal antibody

CLUAP1 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, canine

Applications

WB, IF/ICC, IP, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 17470-1-AP

Print datasheet

Synonyms

Qilin, KIAA0643, Clusterin-associated protein 1, clusterin associated protein 1

验证数据展示

  • WB analysis of mouse testis using 17470-1-AP

    WB analysis of mouse testis using 17470-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 17470-1-AP (CLUAP1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IP experiment of HepG2 using 17470-1-AP

    IP experiment of HepG2 using 17470-1-AP

    IP result of anti-CLUAP1 (IP:17470-1-AP, 4ug; Detection:17470-1-AP 1:600) with HepG2 cells lysate 2400ug.

  • IF Staining of MDCK using 17470-1-AP

    IF Staining of MDCK using 17470-1-AP

    Immunofluorescent analysis of (4% PFA) fixed MDCK cells using CLUAP1 antibody (17470-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CoraLite®594 ARL13B antibody (CL594-17711, red).

经过测试的应用

Positive WB detected inmouse testis tissue, COLO 320 cells, HepG2 cells, Y79 cells
Positive IP detected inHepG2 cells
Positive IF/ICC detected inMDCK cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17470-1-AP targets CLUAP1 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat, canine samples.

Tested Applications WB, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB, IF
Tested Reactivity human, mouse, rat, canine
Cited Reactivityhuman
Immunogen CLUAP1 fusion protein Ag11419 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name clusterin associated protein 1
Synonyms Qilin, KIAA0643, Clusterin-associated protein 1, clusterin associated protein 1
Calculated Molecular Weight 413 aa, 48 kDa
Observed Molecular Weight 53 kDa
GenBank Accession NumberBC017070
Gene Symbol CLUAP1
Gene ID (NCBI) 23059
RRIDAB_2083477
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96AJ1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CLUAP1 (Clusterin-associated protein 1) was originally identified as a protein that interacts with CLU/clusterin, and whose gene is frequently upregulated in colon cancer (PMID: 15480429). Overexpression of CLUAP1 was also observed in osteosarcoma (PMID: 17203229). CLUAP1 is most abundantly expressed in testis among normal tissues (PMID: 15480429; 17203229). Studies reveal that CLUAP1 has a role in ciliogenenesis and cilia-dependent development (PMID: 23351563; 23742838; 24970261).

实验方案

Product Specific Protocols
WB protocol for CLUAP1 antibody 17470-1-APDownload protocol
IF protocol for CLUAP1 antibody 17470-1-APDownload protocol
IP protocol for CLUAP1 antibody 17470-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Dev Cell

The CEP19-RABL2 GTPase Complex Binds IFT-B to Initiate Intraflagellar Transport at the Ciliary Base.

Authors - Tomoharu Kanie
View Article
humanWB,IF

Mol Cell Proteomics

CRISPR/Cas9-mediated genomic editing of Cluap1/IFT38 reveals a new role in actin arrangement.

Authors - Tina Beyer
  • KO Validated
View Article