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- KD/KO Validated
CLUAP1 Polyclonal antibody
CLUAP1 Polyclonal Antibody for IF/ICC, IP, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat, Canine
Applications
IF/ICC, IP, WB,ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | mouse testis tissue, COLO 320 cells, HepG2 cells, Y79 cells |
| Positive IP detected in | HepG2 cells |
| Positive IF detected in | MDCK cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF) | IF : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
17470-1-AP targets CLUAP1 in WB, IP, IF, ELISA applications and shows reactivity with human, mouse, rat, Canine samples.
| Tested Applications | IF/ICC, IP, WB,ELISA |
| Cited Applications | WB, IF |
| Tested Reactivity | human, mouse, rat, Canine |
| Cited Reactivity | human |
| Immunogen | CLUAP1 fusion protein Ag11419 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | clusterin associated protein 1 |
| Synonyms | CLUAP1, clusterin associated protein 1, KIAA0643, Qilin |
| Calculated Molecular Weight | 413 aa, 48 kDa |
| Observed Molecular Weight | 53 kDa |
| GenBank Accession Number | BC017070 |
| Gene Symbol | CLUAP1 |
| Gene ID (NCBI) | 23059 |
| RRID | AB_2083477 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96AJ1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CLUAP1 (Clusterin-associated protein 1) was originally identified as a protein that interacts with CLU/clusterin, and whose gene is frequently upregulated in colon cancer (PMID: 15480429). Overexpression of CLUAP1 was also observed in osteosarcoma (PMID: 17203229). CLUAP1 is most abundantly expressed in testis among normal tissues (PMID: 15480429; 17203229). Studies reveal that CLUAP1 has a role in ciliogenenesis and cilia-dependent development (PMID: 23351563; 23742838; 24970261).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CLUAP1 antibody 17470-1-AP | Download protocol |
| IF protocol for CLUAP1 antibody 17470-1-AP | Download protocol |
| IP protocol for CLUAP1 antibody 17470-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Dev Cell The CEP19-RABL2 GTPase Complex Binds IFT-B to Initiate Intraflagellar Transport at the Ciliary Base. | ||
Mol Cell Proteomics CRISPR/Cas9-mediated genomic editing of Cluap1/IFT38 reveals a new role in actin arrangement.
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