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验证数据展示

  • WB analysis using 31407-1-AP

    WB analysis using 31407-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31407-1-AP (CMIP antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • IHC staining of human intrahepatic cholangiocarcinoma using 31407-1-AP

    IHC staining of human intrahepatic cholangiocarcinoma using 31407-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 31407-1-AP (CMIP antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CMIP antibody included
  • IHC staining of human intrahepatic cholangiocarcinoma using 31407-1-AP

    IHC staining of human intrahepatic cholangiocarcinoma using 31407-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 31407-1-AP (CMIP antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CMIP antibody included

CMIP Polyclonal antibody

CMIP Polyclonal Antibody for WB, IHC, ELISA
Cat No. 31407-1-AP

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

TCMIP, Tc Mip, c-Mip, C-Maf-inducing protein, c Mip

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inRaji cells, MCF-7 cells, K-562 cells, A549 cells
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy CMIP Ready-To-Use IHC Kit. CMIP primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31407-1-AP targets CMIP in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human
Immunogen CMIP fusion protein Ag35679 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name c-Maf-inducing protein
Synonyms TCMIP, Tc Mip, c-Mip, C-Maf-inducing protein, c Mip
Calculated Molecular Weight 86 kDa
Observed Molecular Weight86 kDa
GenBank Accession NumberBC038113
Gene Symbol CMIP
Gene ID (NCBI) 80790
RRIDAB_3669968
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ8IY22
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cmaf‐inducing protein (CMIP) is a newly identified gene that encodes an 86 kDa protein, containing an N‐terminal pleckstrin homology domain (PH), a nuclear localization signal near the PH domain, a middle region characterized by the presence of several interacting docking sites, including a 14‐3‐3 module, a PKC domain, an Erk domain, an SH3 domain similar to the p85 regulatory subunit of phosphatidylinositol 3‐kinase (PI3K), and a C‐terminal leucine‐rich repeat (LRR) domain.

实验方案

Product Specific Protocols
WB protocol for CMIP antibody 31407-1-APDownload protocol
IHC protocol for CMIP antibody 31407-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols