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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated CNN2 Polyclonal antibody

CNN2 Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-21073

Print datasheet

Synonyms

calponin 2, Calponin H2, smooth muscle, CNN2, Neutral calponin

验证数据展示

  • IF Staining of HepG2 using CL488-21073

    IF Staining of HepG2 using CL488-21073

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 CNN2 antibody (CL488-21073) at dilution of 1:200.

经过测试的应用

Positive IF/ICC detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 21073-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-21073 targets CNN2 in IF/ICC applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human, mouse, rat
Immunogen CNN2 fusion protein Ag15225 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name calponin 2
Synonyms calponin 2, Calponin H2, smooth muscle, CNN2, Neutral calponin
Calculated Molecular Weight 309 aa, 34 kDa
Observed Molecular Weight 34-36 kDa
GenBank Accession NumberBC141818
Gene Symbol CNN2
Gene ID (NCBI) 1265
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ99439
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Calponin is a family of actin filament-associated proteins which regulate smooth muscle cell contraction. Three isoforms of calponin exist: calponin h1 (CNN1) , calponin h2 (CNN2) and calponin 3 (CNN3). Calponin 1 and calponin 2 are predominately expressed in smooth muscle cells and cardiac muscle cells, respectively. Calponin 3 is highly expressed in many tissues including articular cartilage and brain.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 CNN2 antibody CL488-21073Download protocol
Standard Protocols
Click here to view our Standard Protocols