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验证数据展示

  • IF Staining of HepG2 using CL488-66507

    IF Staining of HepG2 using CL488-66507

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using CoraLite® Plus 488 CNOT1 antibody (CL488-66507, Clone: 1A10A11 ) at dilution of 1:200.

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated CNOT1 Monoclonal antibody

CNOT1 Monoclonal Antibody for IF/ICC
Cat No. CL488-66507

产品说明书

CloneNo. 1A10A11

宿主/亚型

Mouse / IgG1

种属反应性

Human

应用

IF/ICC

别名

AD 005, CCR4 associated factor 1, CDC39, CNOT1, DKFZp686E0722, DKFZp686O168, FLJ36492, FLJ90644, hNOT1, KIAA1007, NOT1, NOT1H

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy CNOT1 Ready-To-Use IHC Kit. CNOT1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66507-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66507 targets CNOT1 in IF/ICC applications and shows reactivity with Human samples.

经测试应用 IF/ICC Application Description
经测试反应性 Human
免疫原 CNOT1 fusion protein Ag5623 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 CCR4-NOT transcription complex, subunit 1
别名 AD 005, CCR4 associated factor 1, CDC39, CNOT1, DKFZp686E0722, DKFZp686O168, FLJ36492, FLJ90644, hNOT1, KIAA1007, NOT1, NOT1H
计算分子量 267 kDa
观测分子量 240-250 kDa
GenBank蛋白编号BC040523
基因名称 CNOT1
Gene ID (NCBI) 23019
RRIDAB_2883349
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDA5YKK6
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CNOT1 is a component of CCR4-NOT protein complex, which is a regulator of RNA polymerase II transcription, acts as a transcription repressor. CCR4-NOT complex could participate in transcription as well as mRNA degradation. It's highly expressed in brain, heart, thymus, but weak in skeletal muscle and colon. CNOT1 undergoes alternative splicing to produce four isoforms. CNOT1 exsits as many isoforms. The isoform one is about 267 kDa and the isoform four is about 173 kDa.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 CNOT1 antibody CL488-66507Download protocol
Standard Protocols
Click here to view our Standard Protocols