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验证数据展示

  • IF Staining of HepG2 using CL488-66507

    IF Staining of HepG2 using CL488-66507

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using CoraLite® Plus 488 CNOT1 antibody (CL488-66507, Clone: 1A10A11 ) at dilution of 1:200.

  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated CNOT1 Monoclonal antibody

CNOT1 Monoclonal Antibody for IF/ICC
Cat No. CL488-66507

Print datasheet

CloneNo. 1A10A11

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

IF/ICC

Synonyms

AD 005, CCR4 associated factor 1, CDC39, CNOT1, DKFZp686E0722, DKFZp686O168, FLJ36492, FLJ90644, hNOT1, KIAA1007, NOT1, NOT1H

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy CNOT1 Ready-To-Use IHC Kit. CNOT1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66507-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66507 targets CNOT1 in IF/ICC applications and shows reactivity with Human samples.

Tested Applications IF/ICC Application Description
Tested Reactivity Human
Immunogen CNOT1 fusion protein Ag5623 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name CCR4-NOT transcription complex, subunit 1
Synonyms AD 005, CCR4 associated factor 1, CDC39, CNOT1, DKFZp686E0722, DKFZp686O168, FLJ36492, FLJ90644, hNOT1, KIAA1007, NOT1, NOT1H
Calculated Molecular Weight 267 kDa
Observed Molecular Weight 240-250 kDa
GenBank Accession NumberBC040523
Gene Symbol CNOT1
Gene ID (NCBI) 23019
RRIDAB_2883349
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDA5YKK6
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CNOT1 is a component of CCR4-NOT protein complex, which is a regulator of RNA polymerase II transcription, acts as a transcription repressor. CCR4-NOT complex could participate in transcription as well as mRNA degradation. It's highly expressed in brain, heart, thymus, but weak in skeletal muscle and colon. CNOT1 undergoes alternative splicing to produce four isoforms. CNOT1 exsits as many isoforms. The isoform one is about 267 kDa and the isoform four is about 173 kDa.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 CNOT1 antibody CL488-66507Download protocol
Standard Protocols
Click here to view our Standard Protocols