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  • Featured Product
  • KD/KO Validated

CNOT8 Polyclonal antibody, PBS Only

CNOT8 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 10752-1-PBS

Print datasheet

Synonyms

验证数据展示

  • WB analysis of Jurkat using 10752-1-AP (same clone as 10752-1-PBS)

    WB analysis of Jurkat using 10752-1-AP (same clone as 10752-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 10752-1-AP (CNOT8 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10752-1-PBS in a different storage buffer formulation.

  • IHC staining of human prostate cancer using 10752-1-AP (same clone as 10752-1-PBS)

    IHC staining of human prostate cancer using 10752-1-AP (same clone as 10752-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10752-1-AP (CNOT8 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 10752-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CNOT8 antibody included
  • IP experiment of mouse testis using 10752-1-AP (same clone as 10752-1-PBS)

    IP experiment of mouse testis using 10752-1-AP (same clone as 10752-1-PBS)

    IP result of anti-CNOT8 (IP:10752-1-AP, 4ug; Detection:10752-1-AP 1:600) with mouse testis tissue lysate 4000ug. This data was developed using the same antibody clone with 10752-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 10752-1-AP (same clone as 10752-1-PBS)

    IF Staining of HeLa using 10752-1-AP (same clone as 10752-1-PBS)

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 10752-1-AP (CNOT8 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 10752-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy CNOT8 Ready-To-Use IHC Kit. CNOT8 primary antibody included.

产品信息

10752-1-PBS targets CNOT8 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
Tested Reactivity human, mouse
Immunogen CNOT8 fusion protein Ag1201 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name CCR4-NOT transcription complex, subunit 8
Synonyms
Calculated Molecular Weight 34 kDa
Observed Molecular Weight 34 kDa
GenBank Accession NumberBC017366
Gene Symbol CNOT8
Gene ID (NCBI) 9337
RRIDAB_2082470
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UFF9
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CCR4-NOT transcription complex subunit 8(CNOT8), is a ubiquitous transcription factor, which required for a diverse set of processes. The CCR4-NOT complex is a global regulator of RNA polymerase II, and functions as general transcription regulation complex. It consisted part by CCR4, NOT1 to NOT5, and CAF1. This is a rabbit polyclonal antibody raised against the full-length chain of human CNOT8.