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  • KD/KO Validated

CNOT8 Polyclonal antibody

CNOT8 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 10752-1-AP

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA and More (1)

CALIF, CAF2, CAF1-like protein, Caf1b, CAF1 like protein

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -


经过测试的应用

Positive WB detected inJurkat cells, HeLa cells, mouse testis tissue
Positive IP detected inmouse testis tissue
Positive IHC detected inhuman prostate cancer tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy CNOT8 Ready-To-Use IHC Kit. CNOT8 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10752-1-AP targets CNOT8 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB, IF
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Immunogen CNOT8 fusion protein Ag1201 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name CCR4-NOT transcription complex, subunit 8
Synonyms CALIF, CAF2, CAF1-like protein, Caf1b, CAF1 like protein
Calculated Molecular Weight 34 kDa
Observed Molecular Weight 34 kDa
GenBank Accession NumberBC017366
Gene Symbol CNOT8
Gene ID (NCBI) 9337
RRIDAB_2082470
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UFF9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CCR4-NOT transcription complex subunit 8(CNOT8), is a ubiquitous transcription factor, which required for a diverse set of processes. The CCR4-NOT complex is a global regulator of RNA polymerase II, and functions as general transcription regulation complex. It consisted part by CCR4, NOT1 to NOT5, and CAF1. This is a rabbit polyclonal antibody raised against the full-length chain of human CNOT8.

实验方案

Product Specific Protocols
WB protocol for CNOT8 antibody 10752-1-APDownload protocol
IHC protocol for CNOT8 antibody 10752-1-APDownload protocol
IF protocol for CNOT8 antibody 10752-1-APDownload protocol
IP protocol for CNOT8 antibody 10752-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Mol Cell

Mechanism of ribosome-associated mRNA degradation during tubulin autoregulation

Authors - Markus Höpfler
  • KD Validated
humanWB

Mol Biosyst

Large-scale investigation of oxygen response mutants in Saccharomyces cerevisiae.

Authors - Samanfar Bahram B
  • KD Validated
mouseWB

Cells

Translation Efficiency and Degradation of ER-Associated mRNAs Modulated by ER-Anchored poly(A)-Specific Ribonuclease (PARN).

Authors - Tian-Li Duan
humanWB

Nucleic Acids Res

BESST: a novel LncRNA knockout strategy with less genome perturbance

Authors - Shikuan Zhang
humanWB,IF

Cells

Disruption of the Mammalian Ccr4-Not Complex Contributes to Transcription-Mediated Genome Instability

Authors - Nafiseh Chalabi Hagkarim
  • KD Validated
WB

Nat Commun

Maternal mRNA deadenylation is defective in in vitro matured mouse and human oocytes

Authors - Yusheng Liu
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