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CNOT8 Polyclonal antibody

CNOT8 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 10752-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, IP, ELISA and More (1)

CALIF, CAF2, CAF1-like protein, Caf1b, CAF1 like protein

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inJurkat cells, HeLa cells, mouse testis tissue
Positive IP detected inmouse testis tissue
Positive IHC detected inhuman prostate cancer tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy CNOT8 Ready-To-Use IHC Kit. CNOT8 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10752-1-AP targets CNOT8 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB, IF
经测试反应性 human, mouse
文献引用反应性human, mouse
免疫原 CNOT8 fusion protein Ag1201 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 CCR4-NOT transcription complex, subunit 8
别名 CALIF, CAF2, CAF1-like protein, Caf1b, CAF1 like protein
计算分子量 34 kDa
观测分子量 34 kDa
GenBank蛋白编号BC017366
基因名称 CNOT8
Gene ID (NCBI) 9337
RRIDAB_2082470
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UFF9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CCR4-NOT transcription complex subunit 8(CNOT8), is a ubiquitous transcription factor, which required for a diverse set of processes. The CCR4-NOT complex is a global regulator of RNA polymerase II, and functions as general transcription regulation complex. It consisted part by CCR4, NOT1 to NOT5, and CAF1. This is a rabbit polyclonal antibody raised against the full-length chain of human CNOT8.

实验方案

Product Specific Protocols
WB protocol for CNOT8 antibody 10752-1-APDownload protocol
IHC protocol for CNOT8 antibody 10752-1-APDownload protocol
IF protocol for CNOT8 antibody 10752-1-APDownload protocol
IP protocol for CNOT8 antibody 10752-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Mol Cell

Mechanism of ribosome-associated mRNA degradation during tubulin autoregulation

Authors - Markus Höpfler
  • KD Validated
humanWB

Mol Biosyst

Large-scale investigation of oxygen response mutants in Saccharomyces cerevisiae.

Authors - Samanfar Bahram B
  • KD Validated
mouseWB

Cells

Translation Efficiency and Degradation of ER-Associated mRNAs Modulated by ER-Anchored poly(A)-Specific Ribonuclease (PARN).

Authors - Tian-Li Duan
humanWB

Nucleic Acids Res

BESST: a novel LncRNA knockout strategy with less genome perturbance

Authors - Shikuan Zhang
humanWB,IF

Cells

Disruption of the Mammalian Ccr4-Not Complex Contributes to Transcription-Mediated Genome Instability

Authors - Nafiseh Chalabi Hagkarim
  • KD Validated
WB

Nat Commun

Maternal mRNA deadenylation is defective in in vitro matured mouse and human oocytes

Authors - Yusheng Liu
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