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- KD/KO Validated
CNOT8 Polyclonal antibody
CNOT8 Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
IF, IHC, IP, WB, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | Jurkat cells, HeLa cells, mouse testis tissue |
Positive IP detected in | mouse testis tissue |
Positive IHC detected in | human prostate cancer tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HeLa cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
10752-1-AP targets CNOT8 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
Tested Applications | IF, IHC, IP, WB, ELISA |
Cited Applications | IF, WB |
Tested Reactivity | human, mouse |
Cited Reactivity | human, mouse |
Immunogen | CNOT8 fusion protein Ag1201 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | CCR4-NOT transcription complex, subunit 8 |
Synonyms | CAF1, CAF1 like protein, CAF2, CALIF, CALIFp, CCR4 associated factor 8, CNOT8, hCAF1, POP2 |
Calculated Molecular Weight | 34 kDa |
Observed Molecular Weight | 34 kDa |
GenBank Accession Number | BC017366 |
Gene Symbol | CNOT8 |
Gene ID (NCBI) | 9337 |
RRID | AB_2082470 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q9UFF9 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for CNOT8 antibody 10752-1-AP | Download protocol |
IHC protocol for CNOT8 antibody 10752-1-AP | Download protocol |
IF protocol for CNOT8 antibody 10752-1-AP | Download protocol |
IP protocol for CNOT8 antibody 10752-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Mol Cell Mechanism of ribosome-associated mRNA degradation during tubulin autoregulation
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Mol Biosyst Large-scale investigation of oxygen response mutants in Saccharomyces cerevisiae.
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Cells Translation Efficiency and Degradation of ER-Associated mRNAs Modulated by ER-Anchored poly(A)-Specific Ribonuclease (PARN). | ||
Cells Disruption of the Mammalian Ccr4-Not Complex Contributes to Transcription-Mediated Genome Instability
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