验证数据展示
经过测试的应用
| Positive WB detected in | pig cerebellum tissue, U-251 cells, human brain tissue, HepG2 cells, rat brain tissue, rat cerebellum tissue |
| Positive IF-P detected in | mouse brain tissue |
| Positive FC (Intra) detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
| IF | See 1 publications below |
产品信息
66729-1-Ig targets CNPase in WB, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat, pig samples.
| 经测试应用 | WB, IF-P, FC (Intra), ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse, rat, pig |
| 文献引用反应性 | mouse, rat |
| 免疫原 | CNPase fusion protein Ag4227 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | 2',3'-cyclic nucleotide 3' phosphodiesterase |
| 别名 | CNP, EC:3.1.4.37, 2',3'-cyclic-nucleotide 3'-phosphodiesterase, 2',3' cyclic nucleotide 3' phosphodiesterase, 1B9G9 |
| 计算分子量 | 421 aa, 48 kDa |
| 观测分子量 | 45-48 kDa |
| GenBank蛋白编号 | BC028040 |
| 基因名称 | CNPase |
| Gene ID (NCBI) | 1267 |
| RRID | AB_2882079 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P09543 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CNP(2',3'-cyclic-nucleotide 3'-phosphodiesterase) belongs to the cyclic nucleotide phosphodiesterase family. It plays an essential role in axonal survival but not in myelin assembly, coupling oligodendroglial functions in axonal support and myelination. This protein has 2 isoforms produced by alternative splicing with molecular mass of 45 and 48 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CNPase antibody 66729-1-Ig | Download protocol |
| IF protocol for CNPase antibody 66729-1-Ig | Download protocol |
| FC protocol for CNPase antibody 66729-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
CNS Neurosci Ther EAAT3 impedes oligodendrocyte remyelination in chronic cerebral hypoperfusion-induced white matter injury | ||
J Ethnopharmacol Shenzhiling oral liquid protects the myelin sheath against Alzheimer's disease through the PI3K/Akt-mTOR pathway. |
