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  • Featured Product
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CNPase Monoclonal antibody, PBS Only

CNPase Monoclonal Antibody for WB, IF-P, FC (Intra), ELISA

Host / Isotype

Mouse / IgG1

Reactivity

pig, rat, mouse, human

Applications

WB, IF-P, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

1B9G9

Cat no : 66729-1-PBS

Print datasheet

Synonyms

CNP, EC:3.1.4.37, 2',3'-cyclic-nucleotide 3'-phosphodiesterase, 2',3' cyclic nucleotide 3' phosphodiesterase, 1B9G9

验证数据展示

  • WB analysis using 66729-1-Ig (same clone as 66729-1-PBS)

    WB analysis using 66729-1-Ig (same clone as 66729-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66729-1-Ig (CNPase antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66729-1-PBS in a different storage buffer formulation.

  • WB analysis of HepG2 using 66729-1-Ig (same clone as 66729-1-PBS)

    WB analysis of HepG2 using 66729-1-Ig (same clone as 66729-1-PBS)

    WB result of CNPase antibody (66729-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CNPase transfected HepG2 cells. This data was developed using the same antibody clone with 66729-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse brain using 66729-1-Ig (same clone as 66729-1-PBS)

    IF Staining of mouse brain using 66729-1-Ig (same clone as 66729-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 66729-1-Ig (CNPase antibody), at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66729-1-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 66729-1-Ig (same clone as 66729-1-PBS)

    FC experiment of HepG2 using 66729-1-Ig (same clone as 66729-1-PBS)

    1X10^6 HepG2 cells were intracellularly stained with 0.5 ug Anti-Human CNPase (66729-1-Ig, Clone:1B9G9) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.5 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66729-1-PBS in a different storage buffer formulation.

产品信息

66729-1-PBS targets CNPase in WB, IF-P, FC (Intra), ELISA applications and shows reactivity with pig, rat, mouse, human samples.

Tested Applications WB, IF-P, FC (Intra), ELISA Application Description
Tested Reactivity pig, rat, mouse, human
Immunogen CNPase fusion protein Ag4227 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name 2',3'-cyclic nucleotide 3' phosphodiesterase
Synonyms CNP, EC:3.1.4.37, 2',3'-cyclic-nucleotide 3'-phosphodiesterase, 2',3' cyclic nucleotide 3' phosphodiesterase, 1B9G9
Calculated Molecular Weight 421 aa, 48 kDa
Observed Molecular Weight 45-48 kDa
GenBank Accession NumberBC028040
Gene Symbol CNPase
Gene ID (NCBI) 1267
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP09543
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CNP(2',3'-cyclic-nucleotide 3'-phosphodiesterase) belongs to the cyclic nucleotide phosphodiesterase family. It plays an essential role in axonal survival but not in myelin assembly, coupling oligodendroglial functions in axonal support and myelination. This protein has 2 isoforms produced by alternative splicing with molecular mass of 45 and 48 kDa.