• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 66729-1-Ig (same clone as 66729-1-PBS)

    WB analysis using 66729-1-Ig (same clone as 66729-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66729-1-Ig (CNPase antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66729-1-PBS in a different storage buffer formulation.

  • WB analysis of HepG2 using 66729-1-Ig (same clone as 66729-1-PBS)

    WB analysis of HepG2 using 66729-1-Ig (same clone as 66729-1-PBS)

    WB result of CNPase antibody (66729-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CNPase transfected HepG2 cells. This data was developed using the same antibody clone with 66729-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse brain using 66729-1-Ig (same clone as 66729-1-PBS)

    IF Staining of mouse brain using 66729-1-Ig (same clone as 66729-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 66729-1-Ig (CNPase antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66729-1-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 66729-1-Ig (same clone as 66729-1-PBS)

    FC experiment of HepG2 using 66729-1-Ig (same clone as 66729-1-PBS)

    1X10^6 HepG2 cells were intracellularly stained with 0.5 ug Anti-Human CNPase (66729-1-Ig, Clone:1B9G9) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.5 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66729-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

CNPase Monoclonal antibody, PBS Only

CNPase Monoclonal Antibody for WB, IF-P, FC (Intra), ELISA
Cat No. 66729-1-PBS

产品说明书

CloneNo. 1B9G9

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig

应用

WB, IF-P, FC (Intra), ELISA

别名

CNP, EC:3.1.4.37, 2',3'-cyclic-nucleotide 3'-phosphodiesterase, 2',3' cyclic nucleotide 3' phosphodiesterase, 1B9G9

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

产品信息

66729-1-PBS targets CNPase in WB, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, IF-P, FC (Intra), ELISA Application Description
经测试反应性 human, mouse, rat, pig
免疫原 CNPase fusion protein Ag4227 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 2',3'-cyclic nucleotide 3' phosphodiesterase
别名 CNP, EC:3.1.4.37, 2',3'-cyclic-nucleotide 3'-phosphodiesterase, 2',3' cyclic nucleotide 3' phosphodiesterase, 1B9G9
计算分子量 421 aa, 48 kDa
观测分子量 45-48 kDa
GenBank蛋白编号BC028040
基因名称 CNPase
Gene ID (NCBI) 1267
RRIDAB_2882079
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP09543
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CNP(2',3'-cyclic-nucleotide 3'-phosphodiesterase) belongs to the cyclic nucleotide phosphodiesterase family. It plays an essential role in axonal survival but not in myelin assembly, coupling oligodendroglial functions in axonal support and myelination. This protein has 2 isoforms produced by alternative splicing with molecular mass of 45 and 48 kDa.