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COASY Monoclonal antibody, PBS Only

COASY Monoclonal Antibody for Indirect ELISA, IHC, WB

Host / Isotype

Mouse / IgG1

Reactivity

rat, mouse, human

Applications

Indirect ELISA, IHC, WB

Conjugate

Unconjugated

CloneNo.

1D1C11

Cat no : 68358-1-PBS

Print datasheet

Synonyms

Dephosphocoenzyme A kinase, Dephospho-CoA pyrophosphorylase, Dephospho-CoA kinase, Coenzyme A synthase, CoA synthase

验证数据展示

  • WB analysis using 68358-1-Ig (same clone as 68358-1-PBS)

    WB analysis using 68358-1-Ig (same clone as 68358-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68358-1-Ig (COASY antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68358-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 68358-1-Ig (same clone as 68358-1-PBS)

    IHC staining of human liver using 68358-1-Ig (same clone as 68358-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 68358-1-Ig (COASY antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68358-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 68358-1-Ig (same clone as 68358-1-PBS)

    FC experiment of HeLa using 68358-1-Ig (same clone as 68358-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.8 ug COASY Monoclonal antibody (68358-1-Ig, Clone:1D1C11) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.8 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 68358-1-PBS in a different storage buffer formulation.

产品信息

68358-1-PBS targets COASY in Indirect ELISA, IHC, WB applications and shows reactivity with rat, mouse, human samples.

Tested Applications Indirect ELISA, IHC, WB Application Description
Tested Reactivity rat, mouse, human
Immunogen COASY fusion protein Ag33142 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name Coenzyme A synthase
Synonyms Dephosphocoenzyme A kinase, Dephospho-CoA pyrophosphorylase, Dephospho-CoA kinase, Coenzyme A synthase, CoA synthase
Calculated Molecular Weight 564 aa, 62 kDa
Observed Molecular Weight 62 kDa
GenBank Accession NumberBC020985
Gene Symbol COASY
Gene ID (NCBI) 80347
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ13057
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Bifunctional coenzyme A synthase (COASY) is a bifunctional enzyme that catalyzes the fourth and fifth sequential steps of the CoA biosynthetic pathway. The fourth reaction is catalyzed by the phosphopantetheine adenylyltransferase, coded by the coaD domain; the fifth reaction is catalyzed by the dephospho-CoA kinase, coded by the coaE domain. May act as a point of CoA biosynthesis regulation. It was reported that having a key role in erythroid maturation and identified upstream and downstream metabolites of COASY as a potential treatment for anemia in patients with MDS-RS (PMID: 36857430). COASY has 2 isoforms with molecular weights of 62 and 65 kDa.