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Coilin Polyclonal antibody

Coilin Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA and More (2)

Conjugate

Unconjugated

Cat no : 10967-1-AP

Print datasheet

Synonyms

COIL, p80-coilin, p80 coilin, CLN80

验证数据展示

  • WB analysis using 10967-1-AP

    WB analysis using 10967-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10967-1-AP (Coilin antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

  • IHC staining of human prostate cancer using 10967-1-AP

    IHC staining of human prostate cancer using 10967-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10967-1-AP (Coilin antibody) at dilution of 1:100 (under 10x lens).

  • IP experiment of HEK-293 using 10967-1-AP

    IP experiment of HEK-293 using 10967-1-AP

    IP result of anti-Coilin (IP:10967-1-AP, 4ug; Detection:10967-1-AP 1:10000) with HEK-293 cells lysate 1400 ug.

  • IF Staining of HeLa using 10967-1-AP

    IF Staining of HeLa using 10967-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Coilin antibody (10967-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • FC experiment of HEK-293 using 10967-1-AP

    FC experiment of HEK-293 using 10967-1-AP

    1X10^6 HEK-293 cells were intracellularly stained with 0.4 ug Anti-Human Coilin (10967-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive WB detected inHEK-293 cells, human brain tissue, Jurkat cells, K-562 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman prostate cancer tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHEK-293 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488, CoraLite®594 and CoraLite® Plus 647 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10967-1-AP targets Coilin in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description
Cited ApplicationsWB, IHC, CoIP, IF
Tested Reactivity human
Cited Reactivityhuman, mouse
Immunogen Coilin fusion protein Ag1416 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name coilin
Synonyms COIL, p80-coilin, p80 coilin, CLN80
Calculated Molecular Weight 576 aa, 63 kDa
Observed Molecular Weight 80 kDa
GenBank Accession NumberBC010385
Gene Symbol COIL
Gene ID (NCBI) 8161
RRIDAB_2276345
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP38432
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The nuclear coiled body(CBs) and nuclear structures known as gems contain high concentrations of the survival motor neurons (SMN) protein complex. CBs and gems often colocalize, and communication between them is mediated by COIL. COIL is a component of the CBS which are involved in the function or assembly/disassembly of nucleoplasmic snRNPs. During mitosis, CBs disassemble, coinciding with a mitotic-specific phosphorylation of p80 coilin, and symmetrical dimethylation of the COIL RG box motif is a molecular switch that determines whether the SMN complex forms gems or becomes incorporated into CBs

实验方案

Product Specific Protocols
WB protocol for Coilin antibody 10967-1-APDownload protocol
IHC protocol for Coilin antibody 10967-1-APDownload protocol
IF protocol for Coilin antibody 10967-1-APDownload protocol
IP protocol for Coilin antibody 10967-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nucleic Acids Res

mRNAs are sorted for export or degradation before passing through nuclear speckles.

Authors - Jing Fan
View Article
mouseWB,IHC,IF,CoIP

Clin Transl Med

HSF4/COIL complex-dependent R-loop mediates ultraviolet-induced inflammatory skin injury

Authors - Yi-Qian Feng
View Article
humanIF

PLoS Genet

Nuclear Fragile X Mental Retardation Protein Is localized to Cajal Bodies.

Authors - Dury Alain Y AY
View Article
humanIF

Elife

Characterisation of the biflavonoid hinokiflavone as a pre-mRNA splicing modulator that inhibits SENP.

Authors - Andrea Pawellek
View Article
humanWB,IF

Neurobiol Dis

UsnRNP trafficking is regulated by stress granules and compromised by mutant ALS proteins.

Authors - Simona Rossi
View Article
humanWB,IF

Mol Brain

Long non-coding RNA lncC11orf54-1 modulates neuroinflammatory responses by activating NF-κB signaling during meningitic Escherichia coli infection

Authors - Bojie Xu
View Article