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验证数据展示

  • IF Staining of HeLa using CL488-10967

    IF Staining of HeLa using CL488-10967

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 Coilin antibody (CL488-10967) at dilution of 1:200, (CL594-Phalloidin, red).

  • FC experiment of HEK-293T using CL488-10967

    FC experiment of HEK-293T using CL488-10967

    1X10^6 HEK-293T cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human Coilin (CL488-10967) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

CoraLite® Plus 488-conjugated Coilin Polyclonal antibody

Coilin Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-10967

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, FC (Intra)

别名

COIL, p80-coilin, p80 coilin, CLN80

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHEK-293T cells
For other applications, we recommend the unconjugated version of this antibody, 10967-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-10967 targets Coilin in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human
免疫原 Coilin fusion protein Ag1416 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 coilin
别名 COIL, p80-coilin, p80 coilin, CLN80
计算分子量 576 aa, 63 kDa
观测分子量 80 kDa
GenBank蛋白编号BC010385
基因名称 COIL
Gene ID (NCBI) 8161
RRIDAB_2919012
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP38432
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The nuclear coiled body(CBs) and nuclear structures known as gems contain high concentrations of the survival motor neurons (SMN) protein complex. CBs and gems often colocalize, and communication between them is mediated by COIL. COIL is a component of the CBS which are involved in the function or assembly/disassembly of nucleoplasmic snRNPs. During mitosis, CBs disassemble, coinciding with a mitotic-specific phosphorylation of p80 coilin, and symmetrical dimethylation of the COIL RG box motif is a molecular switch that determines whether the SMN complex forms gems or becomes incorporated into CBs

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Coilin antibody CL488-10967Download protocol
FC protocol for CL Plus 488 Coilin antibody CL488-10967Download protocol
Standard Protocols
Click here to view our Standard Protocols