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COL17A1 Polyclonal antibody, PBS Only

COL17A1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 31190-1-PBS

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Synonyms

验证数据展示

  • WB analysis using 31190-1-AP (same clone as 31190-1-PBS)

    WB analysis using 31190-1-AP (same clone as 31190-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 31190-1-AP (COL17A1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31190-1-PBS in a different storage buffer formulation.

  • IHC staining of rat skin using 31190-1-AP (same clone as 31190-1-PBS)

    IHC staining of rat skin using 31190-1-AP (same clone as 31190-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat skin tissue slide using 31190-1-AP (COL17A1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31190-1-PBS in a different storage buffer formulation.

  • IHC staining of rat skin using 31190-1-AP (same clone as 31190-1-PBS)

    IHC staining of rat skin using 31190-1-AP (same clone as 31190-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat skin tissue slide using 31190-1-AP (COL17A1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31190-1-PBS in a different storage buffer formulation.

  • IF Staining of A431 using 31190-1-AP (same clone as 31190-1-PBS)

    IF Staining of A431 using 31190-1-AP (same clone as 31190-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using COL17A1 antibody (31190-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 31190-1-PBS in a different storage buffer formulation.

  • IF Staining of HaCaT using 31190-1-AP (same clone as 31190-1-PBS)

    IF Staining of HaCaT using 31190-1-AP (same clone as 31190-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HaCaT cells using COL17A1 antibody (31190-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 31190-1-PBS in a different storage buffer formulation.

产品信息

31190-1-PBS targets COL17A1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen COL17A1 fusion protein Ag34645 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name collagen, type XVII, alpha 1
Synonyms
Observed Molecular Weight180 kDa
GenBank Accession NumberBC168368
Gene Symbol COL17A1
Gene ID (NCBI) 1308
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ9UMD9
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Collagen alpha-1(XVII) chain, also termed 180-kDa bullous pemphigoid antigen (BP180), is a type II transmembrane glycoprotein with a collagenous carboxyl-terminal extracellular domain and a noncollagenous amino-terminal cytoplasmic domain. High expression of COL17A1 has also been identified as a marker of human esophageal quiescent stem/progenitor cells (PMID: 35709398, 9545306). As a structural component of the hemidesmosome, COL17A1 is not only present as the full-length 180 kDa transmembrane form, but also as a cleaved 120 kDa soluble form that is further processed to a 97 kDa form (PMID: 113594550).