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Collagen Type I Recombinant antibody, PBS Only (Capture)

Collagen Type I Uni-rAbTM Recombinant Antibody for WB, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240665D2

Cat no : 83752-3-PBS

Print datasheet

Synonyms

COL1A2, COL1, Collagen alpha 2(I) chain, col I, Alpha-2 type I collagen

验证数据展示

  • WB analysis of rat skin using 83752-3-RR (same clone as 83752-3-PBS)

    WB analysis of rat skin using 83752-3-RR (same clone as 83752-3-PBS)

    rat skin tissue were subjected to SDS PAGE followed by western blot with 83752-3-RR (COL1A2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83752-3-PBS in a different storage buffer formulation.

  • WB analysis using 83752-3-RR (same clone as 83752-3-PBS)

    WB analysis using 83752-3-RR (same clone as 83752-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83752-3-RR (COL1A2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83752-3-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00716-1

    Cytometric bead array standard curve of MP00716-1

    Cytometric bead array standard curve of MP00716-1, COL1A2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83752-3-PBS. Detection antibody: 83752-1-PBS. Standard: Ag6281. Range: 1.56-100 ng/mL.

  • Cytometric bead array standard curve of MP00716-2

    Cytometric bead array standard curve of MP00716-2

    Cytometric bead array standard curve of MP00716-2, COL1A2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83752-3-PBS. Detection antibody: 83752-2-PBS. Standard: Ag6281. Range: 1.56-100 ng/mL.

  • Affinity and Kinetic Characterization of 83752-3-RR

    Affinity and Kinetic Characterization of 83752-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83752-3-RR against Human Collagen Type I were performed. The affinity constant is 0.777 nM.

产品信息

83752-3-PBS targets Collagen Type I as part of a matched antibody pair:

MP00716-1: 83752-3-PBS capture and 83752-1-PBS detection (validated in Cytometric bead array)

MP00716-2: 83752-3-PBS capture and 83752-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, Cytometric bead array, Indirect ELISA Application Description
Tested Reactivity human, rat
Immunogen Collagen Type I fusion protein Ag6281 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name collagen, type I, alpha 2
Synonyms COL1A2, COL1, Collagen alpha 2(I) chain, col I, Alpha-2 type I collagen
Calculated Molecular Weight 1366 aa, 130 kDa
Observed Molecular Weight100-160 kDa
GenBank Accession NumberBC054498
Gene Symbol COL1A2
Gene ID (NCBI) 1278
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP08123
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Type I collagen, the major structural component of connective tissues such as skin, tendon and bone, is the most abundant and widely expressed collagen in humans (PMID: 7620364; 8645190; 9016532). Type I collagen is a heterotrimer comprising one alpha 2(I) and two alpha 1(I) chains which are encoded by the unlinked loci COL1A2 and COL1A1 respectively. Type I collagen has a molecular mass of about 250-300 kDa, while the alpha 2(I) chain has a molecular weight of about 100-140 kDa. Mutations in COL1A2 gene are associated with osteogenesis imperfecta, Ehlers-Danlos syndrome, idiopathic osteoporosis, and atypical Marfan syndrome. This antibody raised against 1017-1366 aa of human pro-alpha 2 chain of type l collagen can recognize collagen alpha 2(1) chain and C-terminal propeptide of pro-alpha 2(1) chain.