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验证数据展示

  • WB analysis of rat skin using 83752-3-RR (same clone as 83752-3-PBS)

    WB analysis of rat skin using 83752-3-RR (same clone as 83752-3-PBS)

    rat skin tissue were subjected to SDS PAGE followed by western blot with 83752-3-RR (Collagen Type I antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83752-3-PBS in a different storage buffer formulation.

  • WB analysis using 83752-3-RR (same clone as 83752-3-PBS)

    WB analysis using 83752-3-RR (same clone as 83752-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83752-3-RR (COL1A2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83752-3-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00716-1

    Cytometric bead array standard curve of MP00716-1

    Cytometric bead array standard curve of MP00716-1, Collagen Type I Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83752-3-PBS. Detection antibody: 83752-1-PBS. Standard: Ag6281. Range: 1.56-100 ng/mL.

  • Cytometric bead array standard curve of MP00716-2

    Cytometric bead array standard curve of MP00716-2

    Cytometric bead array standard curve of MP00716-2, Collagen Type I Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83752-3-PBS. Detection antibody: 83752-2-PBS. Standard: Ag6281. Range: 1.56-100 ng/mL.

  • Affinity and Kinetic Characterization of 83752-3-PBS

    Affinity and Kinetic Characterization of 83752-3-PBS

    Biolayer interferometry (BLl) kinetic assay of 83752-3-PBS against Human Collagen Type I was performed. The affinity constant is 47.7 pM.

  • Affinity and Kinetic Characterization of 83752-3-RR

    Affinity and Kinetic Characterization of 83752-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83752-3-RR against Human Collagen Type I were performed. The affinity constant is 0.777 nM.

Collagen Type I Recombinant antibody, PBS Only (Capture)

Collagen Type I Uni-rAbTM Recombinant Antibody for WB, Cytometric bead array, Indirect ELISA
Cat No. 83752-3-PBS

产品说明书

CloneNo. 240665D2

宿主/亚型

Rabbit / IgG

种属反应性

human, rat

应用

WB, Cytometric bead array, Indirect ELISA

别名

COL1, COL1A2, 240665D2, Alpha-2 type I collagen, col I

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

83752-3-PBS targets Collagen Type I as part of a matched antibody pair:

MP00716-1: 83752-3-PBS capture and 83752-1-PBS detection (validated in Cytometric bead array)

MP00716-2: 83752-3-PBS capture and 83752-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, rat
免疫原 Collagen Type I fusion protein Ag6281 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 collagen, type I, alpha 2
别名 COL1, COL1A2, 240665D2, Alpha-2 type I collagen, col I
计算分子量 1366 aa, 130 kDa
观测分子量100-160 kDa
GenBank蛋白编号BC054498
基因名称 COL1A2
Gene ID (NCBI) 1278
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP08123
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Type I collagen, the major structural component of connective tissues such as skin, tendon and bone, is the most abundant and widely expressed collagen in humans (PMID: 7620364; 8645190; 9016532). Type I collagen is a heterotrimer comprising one alpha 2(I) and two alpha 1(I) chains which are encoded by the unlinked loci COL1A2 and COL1A1 respectively. Type I collagen has a molecular mass of about 250-300 kDa, while the alpha 2(I) chain has a molecular weight of about 100-140 kDa. Mutations in COL1A2 gene are associated with osteogenesis imperfecta, Ehlers-Danlos syndrome, idiopathic osteoporosis, and atypical Marfan syndrome. This antibody raised against 1017-1366 aa of human pro-alpha 2 chain of type l collagen can recognize collagen alpha 2(1) chain and C-terminal propeptide of pro-alpha 2(1) chain.