Collagen Type I Recombinant antibody

Collagen Type I Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 83752-3-RR

产品说明书

CloneNo. 240665D2

宿主/亚型

Rabbit / IgG

种属反应性

human, rat

应用

WB, ELISA

COL1, COL1A2, 240665D2, Alpha-2 type I collagen, col I

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inMG-63 cells, rat skin tissue, Saos-2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83752-3-RR targets Collagen Type I in WB, ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, rat
免疫原 Collagen Type I fusion protein Ag6281 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 collagen, type I, alpha 2
别名 COL1, COL1A2, 240665D2, Alpha-2 type I collagen, col I
计算分子量 1366 aa, 130 kDa
观测分子量100-160 kDa
GenBank蛋白编号BC054498
基因名称 COL1A2
Gene ID (NCBI) 1278
RRIDAB_3671348
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP08123
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Type I collagen, the major structural component of connective tissues such as skin, tendon and bone, is the most abundant and widely expressed collagen in humans (PMID: 7620364; 8645190; 9016532). Type I collagen is a heterotrimer comprising one alpha 2(I) and two alpha 1(I) chains which are encoded by the unlinked loci COL1A2 and COL1A1 respectively. Type I collagen has a molecular mass of about 250-300 kDa, while the alpha 2(I) chain has a molecular weight of about 100-140 kDa. Mutations in COL1A2 gene are associated with osteogenesis imperfecta, Ehlers-Danlos syndrome, idiopathic osteoporosis, and atypical Marfan syndrome. This antibody raised against 1017-1366 aa of human pro-alpha 2 chain of type l collagen can recognize collagen alpha 2(1) chain and C-terminal propeptide of pro-alpha 2(1) chain.

实验方案

Product Specific Protocols
WB protocol for Collagen Type I antibody 83752-3-RRDownload protocol
Standard Protocols
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