验证数据展示
经过测试的应用
Positive WB detected in | MG-63 cells, rat skin tissue, Saos-2 cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:1000-1:4000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
83752-3-RR targets Collagen Type I in WB, ELISA applications and shows reactivity with human, rat samples.
经测试应用 | WB, ELISA Application Description |
经测试反应性 | human, rat |
免疫原 | Collagen Type I fusion protein Ag6281 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Recombinant |
产品类型 | Antibody |
全称 | collagen, type I, alpha 2 |
别名 | COL1, COL1A2, 240665D2, Alpha-2 type I collagen, col I |
计算分子量 | 1366 aa, 130 kDa |
观测分子量 | 100-160 kDa |
GenBank蛋白编号 | BC054498 |
基因名称 | COL1A2 |
Gene ID (NCBI) | 1278 |
RRID | AB_3671348 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein A purification |
UNIPROT ID | P08123 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Type I collagen, the major structural component of connective tissues such as skin, tendon and bone, is the most abundant and widely expressed collagen in humans (PMID: 7620364; 8645190; 9016532). Type I collagen is a heterotrimer comprising one alpha 2(I) and two alpha 1(I) chains which are encoded by the unlinked loci COL1A2 and COL1A1 respectively. Type I collagen has a molecular mass of about 250-300 kDa, while the alpha 2(I) chain has a molecular weight of about 100-140 kDa. Mutations in COL1A2 gene are associated with osteogenesis imperfecta, Ehlers-Danlos syndrome, idiopathic osteoporosis, and atypical Marfan syndrome. This antibody raised against 1017-1366 aa of human pro-alpha 2 chain of type l collagen can recognize collagen alpha 2(1) chain and C-terminal propeptide of pro-alpha 2(1) chain.
实验方案
Product Specific Protocols | |
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WB protocol for Collagen Type I antibody 83752-3-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |