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Collagen Type III (N-terminal) Recombinant antibody

Collagen Type III (N-terminal) Uni-rAbTM Recombinant Antibody for IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

2O1

Cat no : 80009-1-RR

Print datasheet

Synonyms

COL3A1, COL3, Collagen Type III, Collagen alpha-1(III) chain, Collagen alpha 1(III) chain

验证数据展示

  • IHC staining of human colon using 80009-1-RR

    IHC staining of human colon using 80009-1-RR

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 80009-1-RR (COL3A1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with COL3A1 antibody included
  • IHC staining of human colon using 80009-1-RR

    IHC staining of human colon using 80009-1-RR

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 80009-1-RR (COL3A1 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with COL3A1 antibody included
  • IF Staining of human colon using 80009-1-RR

    IF Staining of human colon using 80009-1-RR

    Immunofluorescent analysis of (4% PFA) fixed human colon tissue using Collagen Type III (N-terminal) antibody (80009-1-RR, Clone: 2O1 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • IF Staining of human colon using 80009-1-RR

    IF Staining of human colon using 80009-1-RR

    Immunofluorescent analysis of (4% PFA) fixed human colon tissue using Collagen Type III (N-terminal) antibody (80009-1-RR, Clone: 2O1 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • IF Staining of human colon using 80009-1-RR

    IF Staining of human colon using 80009-1-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human colon tissue using Collagen Type III (N-terminal) antibody (80009-1-RR, Clone: 2O1 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 80009-1-RR

    Affinity and Kinetic Characterization of 80009-1-RR

    Biolayer interferometry (BLl) kinetic assays of 80009-1-RR against Human Collagen Type III (N-terminal) were performed. The affinity constant is 1.78 nM.

经过测试的应用

Positive IHC detected inhuman colon tissue, human colon cancer tissue, human ovary tumor tissue, human pancreas cancer tissue, human skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman colon tissue
Planning an IHC experiment? We recommend our IHCeasy COL3A1 Ready-To-Use IHC Kit. COL3A1 primary antibody included.

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

80009-1-RR targets Collagen Type III (N-terminal) in IHC, IF-P, ELISA applications and shows reactivity with human samples.

Tested Applications IHC, IF-P, ELISA Application Description
Tested Reactivity human
Immunogen Collagen Type III (N-terminal) fusion protein Ag18658 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name collagen, type III, alpha 1
Synonyms COL3A1, COL3, Collagen Type III, Collagen alpha-1(III) chain, Collagen alpha 1(III) chain
Calculated Molecular Weight 1466 aa, 139 kDa
GenBank Accession NumberBC028178
Gene Symbol COL3A1
Gene ID (NCBI) 1281
RRIDAB_2882939
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP02461
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Type III collagen is a fibrillar forming collagen comprising three α1(III) chains and is expressed in early embryos and throughout embryogenesis (PMID: 9050868). In the adult, type III collagen is a major component of the extracellular matrix in a variety of internal organs and skin. It occurs in most soft connective tissues along with type I collagen (PMID: 2445760). COL3A1 gene encodes type III procollagen. Mutations in this gene are associated with Ehlers-Danlos syndrome types IV, and with aortic and arterial aneurysms (PMID: 10706896; 2243125; 18389341). This antibody was raised against 24-152 aa of prepro α1 (III) chain of human type III procollagen. It can't recognize mouse or rat type III collagen.

实验方案

Product Specific Protocols
IHC protocol for Collagen Type III (N-terminal) antibody 80009-1-RRDownload protocol
IF protocol for Collagen Type III (N-terminal) antibody 80009-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols