CoraLite® Plus 488-conjugated Collagen Type III (N-terminal) Recombinant antibody

Collagen Type III (N-terminal) Uni-rAbTM Recombinant Antibody for IF-P
Cat No. CL488-80009

产品说明书

CloneNo. 2O1

宿主/亚型

Rabbit / IgG

种属反应性

Human

应用

IF-P

COL3A1, Collagen alpha 1(III) chain, collagen, type III, alpha 1, EDS4A, FLJ34534

缓冲液配方:  PBS and Azide
PBS and Azide
规格: 

-/ -


经过测试的应用

Positive IF-P detected inhuman colon tissue
Planning an IHC experiment? We recommend our IHCeasy COL3A1 Ready-To-Use IHC Kit. COL3A1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 80009-1-PBS

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-80009 targets Collagen Type III (N-terminal) in IF-P applications and shows reactivity with Human samples.

经测试应用 IF-P Application Description
经测试反应性 Human
免疫原 Collagen Type III (N-terminal) fusion protein Ag18658 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 collagen, type III, alpha 1
别名 COL3A1, Collagen alpha 1(III) chain, collagen, type III, alpha 1, EDS4A, FLJ34534
计算分子量 1466 aa, 139 kDa
观测分子量 140-180 kDa
GenBank蛋白编号BC028178
基因名称 COL3A1
Gene ID (NCBI) 1281
RRIDAB_2919567
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP02461
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Type III collagen is a fibrillar forming collagen comprising three α1(III) chains and is expressed in early embryos and throughout embryogenesis (PMID: 9050868). In the adult, type III collagen is a major component of the extracellular matrix in a variety of internal organs and skin. It occurs in most soft connective tissues along with type I collagen (PMID: 2445760). COL3A1 gene encodes type III procollagen. Mutations in this gene are associated with Ehlers-Danlos syndrome types IV, and with aortic and arterial aneurysms (PMID: 10706896; 2243125; 18389341). This antibody was raised against 24-152 aa of prepro α1 (III) chain of human type III procollagen. It can't recognize mouse or rat type III collagen.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Collagen Type III (N-terminal) antibody CL488-80009Download protocol
Standard Protocols
Click here to view our Standard Protocols
Loading...