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  • KD/KO Validated

COMMD1 Polyclonal antibody

COMMD1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA and More (2)

Conjugate

Unconjugated

Cat no : 11938-1-AP

Print datasheet

Synonyms

Protein Murr1, MURR1, COMM domain-containing protein 1, C2orf5



经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, mouse brain tissue, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy COMMD1 Ready-To-Use IHC Kit. COMMD1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11938-1-AP targets COMMD1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB, IHC, CoIP, IF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, canine
Immunogen COMMD1 fusion protein Ag2535 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name copper metabolism (Murr1) domain containing 1
Synonyms Protein Murr1, MURR1, COMM domain-containing protein 1, C2orf5
Calculated Molecular Weight 190 aa, 21 kDa
Observed Molecular Weight 21 kDa
GenBank Accession NumberBC022046
Gene Symbol COMMD1
Gene ID (NCBI) 150684
RRIDAB_2083542
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8N668
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

COMMD1(COMM domain-containing protein 1), also named MURR1, is implicated in copper homeostasis by binding one copper ion per monomer. COMMD1 was reported to accelerate the ubiquitination and degradation of NF-kappa-B subunits, its deficiency exerts enhanced NF-kappa-B mediated celluar response. Ubiquitinated by XIAP (X-linked inhibitor of apoptosis), COMMD1 undergoes proteasomal degradation. SOD1 activity can be downregulated by COMMD1.

实验方案

Product Specific Protocols
WB protocol for COMMD1 antibody 11938-1-APDownload protocol
IHC protocol for COMMD1 antibody 11938-1-APDownload protocol
IF protocol for COMMD1 antibody 11938-1-APDownload protocol
IP protocol for COMMD1 antibody 11938-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,mouseWB,IF

Circ Res

COMMD Family Regulates Plasma LDL Levels and Attenuates Atherosclerosis Through Stabilizing the CCC Complex in Endosomal LDLR Trafficking.

Authors - Alina Fedoseienko
  • KD Validated
humanWB

Nat Commun

A genome-wide CRISPR screen identifies host factors that regulate SARS-CoV-2 entry.

Authors - Yunkai Zhu
canineWB,IF

Nat Commun

CCC- and WASH-mediated endosomal sorting of LDLR is required for normal clearance of circulating LDL.

Authors - Paulina Bartuzi
  • KO Validated
human

J Cell Biol

HRS-WASH axis governs actin-mediated endosomal recycling and cell invasion.

Authors - Ewan MacDonald
humanWB,IF,CoIP

Cell Rep

COMMD5/HCaRG Hooks Endosomes on Cytoskeleton and Coordinates EGFR Trafficking.

Authors - Carole G Campion
  • KD Validated
humanWB

Theranostics

SPTBN1 inhibits inflammatory responses and hepatocarcinogenesis via the stabilization of SOCS1 and downregulation of p65 in hepatocellular carcinoma.

Authors - Ling Lin