验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HepG2 cells |
| Positive FC (Intra) detected in | HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy COMMD1 Ready-To-Use IHC Kit. COMMD1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 11938-1-AP
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL594-11938 targets COMMD1 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | IF/ICC, FC (Intra) Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 | COMMD1 fusion protein Ag2535 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | copper metabolism (Murr1) domain containing 1 |
| 别名 | Protein Murr1, MURR1, COMM domain-containing protein 1, C2orf5 |
| 计算分子量 | 190 aa, 21 kDa |
| 观测分子量 | 21 kDa |
| GenBank蛋白编号 | BC022046 |
| 基因名称 | COMMD1 |
| Gene ID (NCBI) | 150684 |
| RRID | AB_2919777 |
| 偶联类型 | CoraLite®594 Fluorescent Dye |
| 最大激发/发射波长 | 588 nm / 604 nm |
| 形式 | Liquid |
| 纯化方式 | Antigen Affinity Purified |
| UNIPROT ID | Q8N668 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
COMMD1(COMM domain-containing protein 1), also named MURR1, is implicated in copper homeostasis by binding one copper ion per monomer. COMMD1 was reported to accelerate the ubiquitination and degradation of NF-kappa-B subunits, its deficiency exerts enhanced NF-kappa-B mediated celluar response. Ubiquitinated by XIAP (X-linked inhibitor of apoptosis), COMMD1 undergoes proteasomal degradation. SOD1 activity can be downregulated by COMMD1.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL594 COMMD1 antibody CL594-11938 | Download protocol |
| FC protocol for CL594 COMMD1 antibody CL594-11938 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |