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COMMD1 Monoclonal antibody, PBS Only

COMMD1 Monoclonal Antibody for WB,Indirect ELISA,IHC,IF

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Pig

Applications

WB,Indirect ELISA,IHC,IF

Conjugate

Unconjugated

CloneNo.

3B10E10

Cat No : 67016-1-PBS

Print datasheet

Synonyms

C2orf5, COMMD1, MURR1, Protein Murr1

验证数据展示

  • WB analysis using 67016-1-Ig (same clone as 67016-1-PBS)

    WB analysis using 67016-1-Ig (same clone as 67016-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67016-1-Ig (COMMD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67016-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 67016-1-Ig (same clone as 67016-1-PBS)

    IHC staining of human breast cancer using 67016-1-Ig (same clone as 67016-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67016-1-Ig (COMMD1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67016-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with COMMD1 antibody included
  • IF Staining of HepG2 using 67016-1-Ig (same clone as 67016-1-PBS)

    IF Staining of HepG2 using 67016-1-Ig (same clone as 67016-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using COMMD1 antibody (67016-1-Ig, Clone: 3B10E10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67016-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy COMMD1 Ready-To-Use IHC Kit. COMMD1 primary antibody included.

产品信息

67016-1-PBS targets COMMD1 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.

Tested Applications WB,Indirect ELISA,IHC,IF Application Description
Tested Reactivity Human, Mouse, Rat, Pig
Immunogen COMMD1 fusion protein Ag28596 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name copper metabolism (Murr1) domain containing 1
Synonyms C2orf5, COMMD1, MURR1, Protein Murr1
Calculated Molecular Weight 190 aa, 21 kDa
Observed Molecular Weight 20 kDa
GenBank Accession NumberBC022046
Gene Symbol COMMD1
Gene ID (NCBI) 150684
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ8N668
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

COMMD1(COMM domain-containing protein 1), also named MURR1, is implicated in copper homeostasis by binding one copper ion per monomer. COMMD1 was reported to accelerate the ubiquitination and degradation of NF-kappa-B subunits, its deficiency exerts enhanced NF-kappa-B mediated celluar response. Ubiquitinated by XIAP (X-linked inhibitor of apoptosis), COMMD1 undergoes proteasomal degradation. SOD1 activity can be downregulated by COMMD1.