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验证数据展示

  • WB analysis using 67016-1-Ig

    WB analysis using 67016-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67016-1-Ig (COMMD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • IHC staining of human breast cancer using 67016-1-Ig

    IHC staining of human breast cancer using 67016-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67016-1-Ig (COMMD1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with COMMD1 antibody included
  • IF Staining of HepG2 using 67016-1-Ig

    IF Staining of HepG2 using 67016-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using COMMD1 antibody (67016-1-Ig, Clone: 3B10E10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

COMMD1 Monoclonal antibody

COMMD1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 67016-1-Ig

产品说明书

CloneNo. 3B10E10

宿主/亚型

Mouse / IgG1

种属反应性

Human, Mouse, Rat, Pig

应用

WB, IHC, IF/ICC, ELISA

别名

C2orf5, COMMD1, MURR1, Protein Murr1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHT-29 cells, A549 cells, HeLa cells, HEK-293 cells, HepG2 cells, HSC-T6 cells, pig brain tissue, rat brain tissue, mouse brain tissue
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy COMMD1 Ready-To-Use IHC Kit. COMMD1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67016-1-Ig targets COMMD1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 Human, Mouse, Rat, Pig
免疫原 COMMD1 fusion protein Ag28596 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 copper metabolism (Murr1) domain containing 1
别名 C2orf5, COMMD1, MURR1, Protein Murr1
计算分子量 190 aa, 21 kDa
观测分子量 20 kDa
GenBank蛋白编号BC022046
基因名称 COMMD1
Gene ID (NCBI) 150684
RRIDAB_2882332
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ8N668
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

COMMD1(COMM domain-containing protein 1), also named MURR1, is implicated in copper homeostasis by binding one copper ion per monomer. COMMD1 was reported to accelerate the ubiquitination and degradation of NF-kappa-B subunits, its deficiency exerts enhanced NF-kappa-B mediated celluar response. Ubiquitinated by XIAP (X-linked inhibitor of apoptosis), COMMD1 undergoes proteasomal degradation. SOD1 activity can be downregulated by COMMD1.

实验方案

Product Specific Protocols
WB protocol for COMMD1 antibody 67016-1-IgDownload protocol
IHC protocol for COMMD1 antibody 67016-1-IgDownload protocol
IF protocol for COMMD1 antibody 67016-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols