• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • IF Staining of HepG2 using CL488-67016

    IF Staining of HepG2 using CL488-67016

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488-conjugated COMMD1 antibody (CL488-67016, Clone: 3B10E10 ) at dilution of 1:100.

  • FC experiment of HepG2 using CL488-67016

    FC experiment of HepG2 using CL488-67016

    1x10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488-conjugated COMMD1 Monoclonal antibody (CL488-67016, Clone:3B10E10)(red), or 0.4 ug CoraLite® Plus 488 Mouse IgG1 Isotype Control (1F8D3) (CL488-66360-1, Clone: 1F8D3) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

CoraLite® Plus 488-conjugated COMMD1 Monoclonal antibody

COMMD1 Monoclonal Antibody for IF/ICC
Cat No. CL488-67016

产品说明书

CloneNo. 3B10E10

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig

应用

IF/ICC

别名

Protein Murr1, MURR1, COMM domain-containing protein 1, C2orf5, 3B10E10

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy COMMD1 Ready-To-Use IHC Kit. COMMD1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67016-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67016 targets COMMD1 in IF/ICC applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 IF/ICC Application Description
经测试反应性 human, mouse, rat, pig
免疫原 COMMD1 fusion protein Ag28596 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 copper metabolism (Murr1) domain containing 1
别名 Protein Murr1, MURR1, COMM domain-containing protein 1, C2orf5, 3B10E10
计算分子量 190 aa, 21 kDa
观测分子量 18 kDa
GenBank蛋白编号BC022046
基因名称 COMMD1
Gene ID (NCBI) 150684
RRIDAB_3672935
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ8N668
储存缓冲液 PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

COMMD1(COMM domain-containing protein 1), also named MURR1, is implicated in copper homeostasis by binding one copper ion per monomer. COMMD1 was reported to accelerate the ubiquitination and degradation of NF-kappa-B subunits, its deficiency exerts enhanced NF-kappa-B mediated celluar response. Ubiquitinated by XIAP (X-linked inhibitor of apoptosis), COMMD1 undergoes proteasomal degradation. SOD1 activity can be downregulated by COMMD1.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 COMMD1 antibody CL488-67016Download protocol
Standard Protocols
Click here to view our Standard Protocols