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COMT Monoclonal antibody, PBS Only

COMT Monoclonal Antibody for WB, IF, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, pig, rat, mouse

Applications

WB, IF, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1A1A4

Cat No : 68181-1-PBS

Print datasheet

Synonyms

catechol O methyltransferase, COMT

验证数据展示

  • WB analysis using 68181-1-Ig (same clone as 68181-1-PBS)

    WB analysis using 68181-1-Ig (same clone as 68181-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68181-1-Ig (COMT antibody) at dilution of 1:10000 and incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68181-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse cerebellum using 68181-1-Ig (same clone as 68181-1-PBS)

    IF Staining of mouse cerebellum using 68181-1-Ig (same clone as 68181-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using COMT antibody (68181-1-Ig, Clone: 1A1A4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68181-1-PBS in a different storage buffer formulation.

产品信息

68181-1-PBS targets COMT in WB, IF, Indirect ELISA applications and shows reactivity with Human, pig, rat, mouse samples.

Tested Applications WB, IF, Indirect ELISA Application Description
Tested Reactivity Human, pig, rat, mouse
Immunogen COMT fusion protein Ag6502 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name catechol-O-methyltransferase
Synonyms catechol O methyltransferase, COMT
Calculated Molecular Weight 30 kDa
Observed Molecular Weight 28-30 kDa
GenBank Accession NumberBC000419
Gene Symbol COMT
Gene ID (NCBI) 1312
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP21964
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

Catechol O-methyltransferase (COMT) is an important enzyme that catalyzes O-methylation and inactivation of metabolically active catechol-containing bioactive molecules and drugs. The mammalian COMT enzyme exists in soluble form (24-26 kDa) and membrane-bound form (28-30 kDa), which are encoded by a single gene utilizing different promoters.