验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, Jurkat cells, NIH/3T3 cells, MCF-7 cells, C2C12 cells |
| Positive IHC detected in | mouse liver tissue, human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
| Positive FC (Intra) detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy COPA Ready-To-Use IHC Kit. COPA primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:125-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
83089-1-RR targets COPA in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IF/ICC, FC (Intra), ELISA Application Description |
| 经测试反应性 | human, mouse |
| 免疫原 | COPA fusion protein Ag34207 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | coatomer protein complex, subunit alpha |
| 别名 | HEP COP, FLJ26320, Coatomer subunit alpha, Alpha COP, Alpha coat protein |
| 计算分子量 | 138 kDa |
| 观测分子量 | 150 kDa |
| GenBank蛋白编号 | NM_004371 |
| 基因名称 | COPA |
| Gene ID (NCBI) | 1314 |
| RRID | AB_3670804 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P53621 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
COPA, also named HEP-COP and Alpha-COP, is coatomer subunit alpha. The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for COPA antibody 83089-1-RR | Download protocol |
| IHC protocol for COPA antibody 83089-1-RR | Download protocol |
| IF protocol for COPA antibody 83089-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |