COPG Polyclonal antibody
COPG Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, IP, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | K-562 cells, A375 cells, HeLa cells, human brain tissue, human colon tissue, NIH/3T3 cells |
Positive IP detected in | K-562 cells |
Positive IHC detected in | mouse kidney tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HepG2 cells, HeLa cells, NIH/3T3 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
12393-1-AP targets COPG in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | WB, IHC, IF/ICC, IP, ELISA Application Description |
Cited Applications | WB, IF, IP |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse |
Immunogen | COPG fusion protein Ag3070 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | coatomer protein complex, subunit gamma |
Synonyms | Gamma-1-COP, Gamma-1-coat protein, COPG1, Coatomer subunit gamma-1 |
Calculated Molecular Weight | 874 aa, 98 kDa |
Observed Molecular Weight | 98 kDa |
GenBank Accession Number | BC020498 |
Gene Symbol | COPG |
Gene ID (NCBI) | 22820 |
RRID | AB_2081435 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q9Y678 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
Product Specific Protocols | |
---|---|
WB protocol for COPG antibody 12393-1-AP | Download protocol |
IHC protocol for COPG antibody 12393-1-AP | Download protocol |
IF protocol for COPG antibody 12393-1-AP | Download protocol |
IP protocol for COPG antibody 12393-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Nat Commun Deficiency in coatomer complex I causes aberrant activation of STING signalling. | ||
J Exp Med Trapping of CDC42 C-terminal variants in the Golgi drives pyrin inflammasome hyperactivation. | ||
J Biol Chem Hepatic loss ofLissencephaly 1induces fatty liver and accelerates liver tumorigenesis in mice. | ||
Hum Mol Genet Quantitative proteomics identifies proteins that resist translational repression and become dysregulated in ALS-FUS. | ||