COPG Polyclonal antibody
COPG Polyclonal Antibody for IF/ICC, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
IF/ICC, IHC, IP, WB, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | K-562 cells, A375 cells, HeLa cells, human brain tissue, human colon tissue, NIH/3T3 cells |
| Positive IP detected in | K-562 cells |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HepG2 cells, NIH/3T3 cells, HeLa cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF) | IF : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
12393-1-AP targets COPG in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | IF/ICC, IHC, IP, WB, ELISA Application Description |
| Cited Applications | WB, IP, IF |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Immunogen | COPG fusion protein Ag3070 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | coatomer protein complex, subunit gamma |
| Synonyms | Coatomer subunit gamma, COPG, COPG1, Gamma coat protein, Gamma COP |
| Calculated Molecular Weight | 874 aa, 98 kDa |
| Observed Molecular Weight | 98 kDa |
| GenBank Accession Number | BC020498 |
| Gene Symbol | COPG |
| Gene ID (NCBI) | 22820 |
| RRID | AB_2081435 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9Y678 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for COPG antibody 12393-1-AP | Download protocol |
| IHC protocol for COPG antibody 12393-1-AP | Download protocol |
| IF protocol for COPG antibody 12393-1-AP | Download protocol |
| IP protocol for COPG antibody 12393-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun Deficiency in coatomer complex I causes aberrant activation of STING signalling. | ||
J Exp Med Trapping of CDC42 C-terminal variants in the Golgi drives pyrin inflammasome hyperactivation. | ||
J Biol Chem Hepatic loss ofLissencephaly 1induces fatty liver and accelerates liver tumorigenesis in mice. | ||
Hum Mol Genet Quantitative proteomics identifies proteins that resist translational repression and become dysregulated in ALS-FUS. | ||
