COPG Polyclonal antibody

COPG Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 12393-1-AP

Print datasheet

Synonyms

Gamma-1-COP, Gamma-1-coat protein, COPG1, Coatomer subunit gamma-1



经过测试的应用

Positive WB detected inK-562 cells, A375 cells, HeLa cells, human brain tissue, human colon tissue, NIH/3T3 cells
Positive IP detected inK-562 cells
Positive IHC detected inmouse kidney tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells, HeLa cells, NIH/3T3 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12393-1-AP targets COPG in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB, IF, IP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen COPG fusion protein Ag3070 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name coatomer protein complex, subunit gamma
Synonyms Gamma-1-COP, Gamma-1-coat protein, COPG1, Coatomer subunit gamma-1
Calculated Molecular Weight 874 aa, 98 kDa
Observed Molecular Weight 98 kDa
GenBank Accession NumberBC020498
Gene Symbol COPG
Gene ID (NCBI) 22820
RRIDAB_2081435
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y678
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for COPG antibody 12393-1-APDownload protocol
IHC protocol for COPG antibody 12393-1-APDownload protocol
IF protocol for COPG antibody 12393-1-APDownload protocol
IP protocol for COPG antibody 12393-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Nat Commun

Deficiency in coatomer complex I causes aberrant activation of STING signalling.

Authors - Annemarie Steiner
IF

J Exp Med

Trapping of CDC42 C-terminal variants in the Golgi drives pyrin inflammasome hyperactivation.

Authors - Masahiko Nishitani-Isa
mouseWB

J Biol Chem

Hepatic loss ofLissencephaly 1induces fatty liver and accelerates liver tumorigenesis in mice.

Authors - Xiaoling Li
mouseWB

Hum Mol Genet

Quantitative proteomics identifies proteins that resist translational repression and become dysregulated in ALS-FUS.

Authors - Desiree M Baron
humanIP,IF,WB

Sci Adv

HPV is a cargo for the COPI sorting complex during virus entry

Authors - Mara C Harwood