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  • Featured Product
  • KD/KO Validated

COPS6 Polyclonal antibody, PBS Only

COPS6 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig, monkey

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 10256-1-PBS

Print datasheet

Synonyms

验证数据展示

  • IHC staining of human colon using 10256-1-AP (same clone as 10256-1-PBS)

    IHC staining of human colon using 10256-1-AP (same clone as 10256-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10256-1-AP (COPS6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10256-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with COPS6 antibody included
  • WB analysis using 10256-1-AP (same clone as 10256-1-PBS)

    WB analysis using 10256-1-AP (same clone as 10256-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 10256-1-AP (COPS6 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10256-1-PBS in a different storage buffer formulation.

  • WB analysis of 3T3-L1 using 10256-1-AP (same clone as 10256-1-PBS)

    WB analysis of 3T3-L1 using 10256-1-AP (same clone as 10256-1-PBS)

    3T3-L1 cells were subjected to SDS PAGE followed by western blot with 10256-1-AP (COPS6 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10256-1-PBS in a different storage buffer formulation.

  • IF Staining of COLO 320 using 10256-1-AP (same clone as 10256-1-PBS)

    IF Staining of COLO 320 using 10256-1-AP (same clone as 10256-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed COLO 320 cells using COPS6 antibody (10256-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 10256-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 10256-1-AP (same clone as 10256-1-PBS)

    IF Staining of HeLa using 10256-1-AP (same clone as 10256-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using COPS6 antibody (10256-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 10256-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy COPS6 Ready-To-Use IHC Kit. COPS6 primary antibody included.

产品信息

10256-1-PBS targets COPS6 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig, monkey samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat, pig, monkey
Immunogen COPS6 fusion protein Ag0331 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name COP9 constitutive photomorphogenic homolog subunit 6 (Arabidopsis)
Synonyms
Calculated Molecular Weight 327 aa, 36 kDa
Observed Molecular Weight34 kDa
GenBank Accession NumberBC002520
Gene Symbol COPS6
Gene ID (NCBI) 10980
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ7L5N1
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The COPS6 protein, also named mov34, is a 34 kDa subunit of COP9 signalosome, a highly conserved eight-subunit protein complex that functions as an important regulator in multiple signaling pathways (PMID: 38308184). This protein belongs to translation initiation factor 3 (eIF3) superfamily. It is involved in the regulation of cell cycle and acts as the regulatory subunit of the 26S proteasome.