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验证数据展示

  • WB analysis using 83312-1-RR

    WB analysis using 83312-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83312-1-RR (COPS8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human intrahepatic cholangiocarcinoma using 83312-1-RR

    IHC staining of human intrahepatic cholangiocarcinoma using 83312-1-RR

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 83312-1-RR (COPS8 antibody) at dilution of 1:250 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human intrahepatic cholangiocarcinoma using 83312-1-RR

    IHC staining of human intrahepatic cholangiocarcinoma using 83312-1-RR

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 83312-1-RR (COPS8 antibody) at dilution of 1:250 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of SH-SY5Y using 83312-1-RR

    IF Staining of SH-SY5Y using 83312-1-RR

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using COPS8 antibody (83312-1-RR, Clone: 240195B1 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 83312-1-RR

    Affinity and Kinetic Characterization of 83312-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83312-1-RR against Human COPS8 were performed. The affinity constant is 1.67 nM.

COPS8 Recombinant antibody

COPS8 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 83312-1-RR
CloneNo. 240195B1

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

COP9, 240195B1, JAB1-containing signalosome subunit 8, hCOP9, CSN8

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inSH-SY5Y cells, HepG2 cells, T-47D cells, NIH/3T3 cells, mouse brain tissue, rat brain tissue
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inSH-SY5Y cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:125-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83312-1-RR targets COPS8 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen COPS8 fusion protein Ag0143 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name COP9 constitutive photomorphogenic homolog subunit 8 (Arabidopsis)
Synonyms COP9, 240195B1, JAB1-containing signalosome subunit 8, hCOP9, CSN8
Calculated Molecular Weight 23 kDa
Observed Molecular Weight23 kDa
GenBank Accession NumberBC003090
Gene Symbol COPS8
Gene ID (NCBI) 10920
RRIDAB_3670979
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ99627
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

COPS8 is one of the eight subunits of COP9 signalosome, a highly conserved protein complex that functions as an important regulator in multiple signaling pathways. The structure and function of COP9 signalosome is similar to that of the 19S regulatory particle of 26S proteasome. COP9 signalosome has been shown to interact with SCF-type E3 ubiquitin ligases and act as a positive regulator of E3 ubiquitin ligases.

实验方案

Product Specific Protocols
WB protocol for COPS8 antibody 83312-1-RRDownload protocol
IHC protocol for COPS8 antibody 83312-1-RRDownload protocol
IF protocol for COPS8 antibody 83312-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols