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验证数据展示

  • IF Staining of HepG2 using CL594-60237

    IF Staining of HepG2 using CL594-60237

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CL594-60237 (COTL1 antibody) at dilution of 1:100.

  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated COTL1 Monoclonal antibody

COTL1 Monoclonal Antibody for IF/ICC
Cat No. CL594-60237

Print datasheet

CloneNo. 4A3D9

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, pig

Applications

IF/ICC

Synonyms

CLP, Coactosin like protein, COTL1, FLJ43657

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite®594
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 60237-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-60237 targets COTL1 in IF/ICC applications and shows reactivity with human, mouse, pig samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human, mouse, pig
Immunogen COTL1 fusion protein Ag1230 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name coactosin-like 1 (Dictyostelium)
Synonyms CLP, Coactosin like protein, COTL1, FLJ43657
Calculated Molecular Weight 16 kDa
Observed Molecular Weight 16 kDa
GenBank Accession NumberBC010884
Gene Symbol COTL1
Gene ID (NCBI) 23406
RRIDAB_2883448
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ14019
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

COTL1, also named as CLP, binds to F-actin in a calcium-independent manner. And it has no direct effect on actin depolymerization.

实验方案

Product Specific Protocols
IF protocol for CL594 COTL1 antibody CL594-60237Download protocol
Standard Protocols
Click here to view our Standard Protocols