验证数据展示
经过测试的应用
Positive IF-P detected in | mouse lung tissue |
推荐稀释比
应用 | 推荐稀释比 |
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Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL594-66351 targets COX2/ Cyclooxygenase 2/ PTGS2 in IF-P applications and shows reactivity with human samples.
经测试应用 | IF-P Application Description |
经测试反应性 | human |
免疫原 | COX2/ Cyclooxygenase 2/ PTGS2 fusion protein Ag24721 种属同源性预测 |
宿主/亚型 | Mouse / IgG2a |
抗体类别 | Monoclonal |
产品类型 | Antibody |
全称 | prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase) |
别名 | COX 2, COX2, COX2/ Cyclooxygenase 2/ PTGS2, Cyclooxygenase 2, GRIPGHS, hCox 2, PGG/HS, PGH synthase 2, PGHS 2, PHS 2, PHS II, Prostaglandin G/H synthase 2, Prostaglandin H2 synthase 2, PTGS2 |
计算分子量 | 604 aa, 68 kDa |
观测分子量 | 66-70 kDa |
GenBank蛋白编号 | BC013734 |
基因名称 | COX2/PTGS2 |
Gene ID (NCBI) | 5743 |
RRID | AB_3673500 |
偶联类型 | CoraLite®594 Fluorescent Dye |
最大激发/发射波长 | 588 nm / 604 nm |
形式 | Liquid |
纯化方式 | Protein A purification |
UNIPROT ID | P35354 |
储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
COX2 (Prostaglandin G/H synthase 2, PTGS2) mediates the formation of prostaglandins from arachidonate. Its subunit structure is homodimer. The fully N-glycosylated PTGS2 is 72-74 kDa and the aglycosylated is 66 kDa (PMID:19656660). It also expresses a band of 39 kDa after unspecific cleavage (PMID:17509125). The 50 kDa band of fragmented PTGS2 has also previously been detected in AD brains (PMID:14724276).
实验方案
Product Specific Protocols | |
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IF protocol for CL594 COX2/ Cyclooxygenase 2/ PTGS2 antibody CL594-66351 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |