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  • Featured Product
  • KD/KO Validated

COX4I2 Polyclonal antibody, PBS Only

COX4I2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 11463-1-PBS

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Synonyms

验证数据展示

  • WB analysis using 11463-1-AP (same clone as 11463-1-PBS)

    WB analysis using 11463-1-AP (same clone as 11463-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 11463-1-AP (COX4I2 antibody) at dilution of 1:1500 incubated at 4 degree celsius over night. This data was developed using the same antibody clone with 11463-1-PBS in a different storage buffer formulation.

  • IP experiment using 11463-1-AP (same clone as 11463-1-PBS)

    IP experiment using 11463-1-AP (same clone as 11463-1-PBS)

    IP result of anti-COX4I2 (11463-1-AP for IP and Detection) with HepG2 cell lysate. This data was developed using the same antibody clone with 11463-1-PBS in a different storage buffer formulation.

  • IP experiment of HepG2 using 11463-1-AP (same clone as 11463-1-PBS)

    IP experiment of HepG2 using 11463-1-AP (same clone as 11463-1-PBS)

    IP result of anti-COX4I2 (IP:11463-1-AP, 3ug; Detection:11463-1-AP 1:1000) with HepG2 cells lysate 8000ug. This data was developed using the same antibody clone with 11463-1-PBS in a different storage buffer formulation.

  • IHC staining of human ovary tumor using 11463-1-AP (same clone as 11463-1-PBS)

    IHC staining of human ovary tumor using 11463-1-AP (same clone as 11463-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor using 11463-1-AP (COX4I2 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11463-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 11463-1-AP (same clone as 11463-1-PBS)

    IF Staining of HeLa using 11463-1-AP (same clone as 11463-1-PBS)

    Immunofluorescent analysis of Hela cells, using COX4I2 antibody 11463-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye). This data was developed using the same antibody clone with 11463-1-PBS in a different storage buffer formulation.

产品信息

11463-1-PBS targets COX4I2 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen COX4I2 fusion protein Ag1987 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name cytochrome c oxidase subunit IV isoform 2 (lung)
Synonyms
Calculated Molecular Weight 171 aa, 20 kDa
Observed Molecular Weight 17-18 kDa
GenBank Accession NumberBC057779
Gene Symbol COX4I2
Gene ID (NCBI) 84701
RRIDAB_2085287
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96KJ9
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

COX4I2, also named as COX4L2 and COXIV-2, belongs to the cytochrome c oxidase IV family. It is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport. COXIV(cytochrome c oxidase IV) has two isoforms (isoform 1 and 2). Isoform 1(COX4I1) is ubiquitously expressed and isoform 2 is highly expressed in lung tissues. This antibody was generated against full length COX4I2 protein.