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验证数据展示

  • WB analysis using 68826-1-Ig

    WB analysis using 68826-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68826-1-Ig (CPA1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of mouse pancreas using 68826-1-Ig

    IF Staining of mouse pancreas using 68826-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse pancreas tissue using CPA1 antibody (68826-1-Ig, Clone: 2H2E8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CPA1 Monoclonal antibody

CPA1 Monoclonal Antibody for WB, IF-P, ELISA
Cat No. 68826-1-Ig

产品说明书

CloneNo. 2H2E8

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig

应用

WB, IF-P, ELISA

别名

Carboxypeptidase A1, EC:3.4.17.1, CPA, 2H2E8

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inpig pancreas tissue, rat pancreas tissue, mouse pancreas tissue
Positive IF-P detected inmouse pancreas tissue
Planning an IHC experiment? We recommend our IHCeasy CPA1 Ready-To-Use IHC Kit. CPA1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68826-1-Ig targets CPA1 in WB, IF-P, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, IF-P, ELISA Application Description
经测试反应性 human, mouse, rat, pig
免疫原 CPA1 fusion protein Ag8579 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 carboxypeptidase A1 (pancreatic)
别名 Carboxypeptidase A1, EC:3.4.17.1, CPA, 2H2E8
计算分子量 419 aa, 47 kDa
观测分子量47 kDa
GenBank蛋白编号BC005279
基因名称 Carboxypeptidase A1
Gene ID (NCBI) 1357
RRIDAB_3670437
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP15085
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CPA1(Carboxypeptidase A1) is also named as CPA and belongs to the the peptidase M14 family. CPA1 is one of the genes whose expression in the pancreas and the protein which it encodes is synthesized as an inactive precursor, proCPA1, which is processed to the active enzyme by the proteolytic removal of the 95-amino acid N-terminal prodomain. The mature CPA1 is not secreted and is trapped and degraded intracellularly(PMID:8639628). This antibody is speicific to CPA1.

实验方案

Product Specific Protocols
WB protocol for CPA1 antibody 68826-1-IgDownload protocol
IF protocol for CPA1 antibody 68826-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols