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CPA1 Monoclonal antibody, PBS Only

CPA1 Monoclonal Antibody for WB, IF-P, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

2H2E8

Cat no : 68826-1-PBS

Print datasheet

Synonyms

Carboxypeptidase A1, EC:3.4.17.1, CPA, 2H2E8

验证数据展示

  • WB analysis using 68826-1-Ig (same clone as 68826-1-PBS)

    WB analysis using 68826-1-Ig (same clone as 68826-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68826-1-Ig (CPA1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68826-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse pancreas using 68826-1-Ig (same clone as 68826-1-PBS)

    IF Staining of mouse pancreas using 68826-1-Ig (same clone as 68826-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse pancreas tissue using CPA1 antibody (68826-1-Ig, Clone: 2H2E8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68826-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy CPA1 Ready-To-Use IHC Kit. CPA1 primary antibody included.

产品信息

68826-1-PBS targets CPA1 in WB, IF-P, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Applications WB, IF-P, ELISA Application Description
Tested Reactivity human, mouse, rat, pig
Immunogen CPA1 fusion protein Ag8579 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name carboxypeptidase A1 (pancreatic)
Synonyms Carboxypeptidase A1, EC:3.4.17.1, CPA, 2H2E8
Calculated Molecular Weight 419 aa, 47 kDa
Observed Molecular Weight47 kDa
GenBank Accession NumberBC005279
Gene Symbol Carboxypeptidase A1
Gene ID (NCBI) 1357
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP15085
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CPA1(Carboxypeptidase A1) is also named as CPA and belongs to the the peptidase M14 family. CPA1 is one of the genes whose expression in the pancreas and the protein which it encodes is synthesized as an inactive precursor, proCPA1, which is processed to the active enzyme by the proteolytic removal of the 95-amino acid N-terminal prodomain. The mature CPA1 is not secreted and is trapped and degraded intracellularly(PMID:8639628). This antibody is speicific to CPA1.